登革热病毒SCID-LO2人鼠嵌合模型的建立与评价.PDFVIP

·413 · ·专题报道· 登革热病毒SCID-LO2 人鼠嵌合模型的 建立与评价 盛子洋，陈艳雷，王娟，吴娜，范东瀛，王湘，高娜，安静 首都医科大学基础医学院微生物教研室，北京 100069 摘要：目的 构建登革热病毒（DENV）感染的小动物模型，为阐明其致病机制提供实验材料。方法 向严重联合免疫缺 陷（SCID）小鼠腹腔内接种人正常肝细胞（LO2 ）构建“人鼠嵌合体”动物模型，进而腹腔注射DENV ，通过检查病毒在体内 分布和主要器官的组织学改变，对DENV 感染的动物模型进行评价。结果 SCID 小鼠成功移植LO2 ，并且嵌合小鼠感染 DENV 后出现病毒血症及严重的器官损伤等表现，但无后肢麻痹等无关症状。结论 成功构建了SCID-LO2 人鼠嵌合模 型，嵌合小鼠能够支持DENV 复制，并表现出部分人类感染DENV 的临床症状，该小鼠感染模型为研究DENV 的致病机制 提供了有价值的实验材料。 关键词：登革热病毒；动物模型；人正常肝细胞株 + 中图分类号：R373.3 3 文献标志码：A 文章编号：1003-4692（2012 ）05-0413-04 Development and evaluation of Dengue virus⁃infected SCID-LO2 chimeric mouse model SHENG Zi⁃yang, CHEN Yan⁃lei, WANG Juan, WU Na, FAN Dong⁃ying, WANG Xiang, GAO Na, AN Jing Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China Corresponding authors: GAO Na, Email: gaona1979@; AN Jing, Email: anjing@ Supported by the National Natural Science Foundation of China （No. Abstract: Objective To develop a mouse model for exploration of the pathogenesis of Dengue virus (DENV) infection. Methods Human normal hepatic cell line LO2 was transplanted to severe combined immunodeficiency (SCID) mice intraperitoneally to develop a SCID-LO2 chimeric mouse model. Following challenge with intraperitoneally injected DENV, the model was evaluated based on virus distribution and the histological changes of main organs. Results The LO2 cells were successfully engrafted into SCID mice. SCID-LO2 chimeric mice showed viremia and severe orga