创伤性休克兔肺组织IL医学论文.docVIP

创伤性休克兔肺组织IL 】目的观察创伤性休克兔肺组织白细胞介素18 (interleukin- 18,IL-18 超氧化物歧化酶 ( superoxidedismutase,SOD ) （malondialdehyde,MDA）水平的变化及乌苷酸环化酶抑制剂亚甲 蓝（methylene blue，MB）的干预作用。方法选用大白兔18只，随 机分为对照组（6只）、创伤性休克生理盐水复苏组（生理盐水复苏组， 6只）、创伤性休克MB处理组（MB处理组，6只），生理盐水复苏 组及MB处理组测定复苏后4 h肺组织IL-18、SOD、MDA的水平, 对照组测定实验末肺组织IL-18、SOD、MDA的水平。结果兔创 伤性休克复苏后肺组织IL-18及MDA明显升高，SOD明显降低， 但MB处理组肺组织IL-18及MDA含量明显低于生理盐水复苏组， SOD水平明显高于生理盐水复苏组。结论IL-18及氧自由基在创伤 性休克的病理发展过程中起着重要作用，应用MB可降低肺组织 IL-18及MDA的水平，增加SOD含量，对创伤性休克缺血/再灌注 肺损伤有保护作用。 关键词】休克创伤性白细胞介素18超氧化物歧化酶丙二醛 亚甲蓝 Abstract: Objective To observe the changes of lung tissue interleukin-18 (IL-18), superoxidedismutase (SOD) and malondialdehyde (MDA) in rabbit models of traumatic shock and the intervention with methylene blue (MB，a guanylate cyclase inhibitor). Methods Eighteen rabbits were randomly assigned into 3 groups (n=6 each)，namely the normal control group, the traumatic shock resuscitated with normal saline group (NS group) and the traumatic shock resuscitated with methylene blue group (MB group). Four hours after resuscitation or by the end of the experiment, lung tissue was taken to measure the contents of IL-18, SOD and MDA. Results In the NS and MB shock groups, the levels of lung tissue IL-18 and MDA were increased and the level of lung tissue SOD was obviously decreased after resuscitation, as compared with that in the control group. But the levels of IL-18 and MDA were obviously lower and the level of SOD was higher in MB group than that in NS group. Conclusions IL-18 and oxygen free radicals play important roles in the pathological process of traumatic shock，and the use of MB in resuscitation can remarkably alleviate the lung injury after traumatic shock by inhibiting IL-18 and MDA production and increasing SOD content. Key words: traumatic shock; interleukin-18; superoxidedismutase; malondialdehyde; methylene blue 创伤性休克是临床上常见的休克类型，是导致多器官功能障碍综 合征发生发展的重要原因之一。急性肺损伤是休克后全身炎性反应综 合征的一个严重阶段，因此，休克后肺保护相当重要。本实验通过建 立创伤性休克及复苏模型，观察创伤性休克兔复苏后及亚甲蓝 （methylene blue,MB）干预后肺组织白细胞介素1