应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清蛋白表达谱.doc

应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清蛋白表达谱 李 莉1，谌宏鸣2 （新疆医科大学：1附属肿瘤医院妇科，乌鲁木齐 830011，2基础学院组织胚胎教研室，乌鲁木齐 830054） 提要：目的 应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清蛋白表达谱，寻找具有潜在诊断意义的血清标志物,以利于胃癌、肝癌的早期诊断。方法 收集1组胃癌患者 (20例)和1组肝癌患者 (10例)以及 1组正常人(20例)血清样本,经ClinProt磁珠纯化、基质辅助激光解析电离飞行时间质谱 (MALDI–TOF–MS)分析及ClinProTools生物信息学方法研究其血清蛋白表达谱。结果 质荷比(m/z)为2863和4965的蛋白峰能较好地鉴别胃癌和非癌组。m/z为1618和4965的蛋白峰能较好地鉴别肝癌和非癌组。质荷比m/z为4965的蛋白峰在胃癌、肝癌中较正常人均异常高表达。结论 液体蛋白芯片飞行时间质谱系统作为研究蛋白表达谱的工具,具有较好的重复性与精度,筛选得到的m/z为2863、1618和4965的蛋白可能为潜在的胃癌、肝癌肿瘤标志物。 　　 关键词：胃癌；肝癌；液体蛋白芯片飞行质谱；蛋白质谱；早期诊断 　　 Proteomic profiling of gastric cancer and hepatic cancer in serum using magnetic bead based sample fractionation and MALDI-TOF- MS LI Li1，CHEN Hong-ming2 （1Department of Gynecology，Affiliated Tumor Hospital, Xinjiang Medical University , Urumqi 830011, 2Department of Histology and Embryology，Xinjiang Medical University , Urumqi 830054） Abstract: Objective To establish proteomic profiling using functional magnetic bead based sample fractionation method combined with matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS) technology and to seek potential biomarkers of gastric cancer and hepatic cancer for early diagnosis. Methods The plasma protein profiling of three groups including 20 gastric cancer patients and 10 hepatic caner patients and 20 normal controls by the ClinProt magnetic bead enrichment and MALDI-TOF-MS was analyzed. Results Two peaks with mass charge ratio (m/z) 2863 and 4965 optimally discriminated gastric cancer from non-cancer control group. m/z 1618 and 4965 optimally discriminated hepatic cancer from non-cancer control group. m/z 4965 was up-regulated in two cancers. Conclusions Functional magnetic bead based sample fractionation method combined with MALDI-TOF-MS is accurate and stable. Protein peaks with m / z 2863 and 1618 and 4965 may be potential biomarkers for gastric cancer and hepatic cancer. 　　Key words: gastric cancer; hepatic cancer; ClinProt system; Proteomic profiling; Early diagnosis