反义rtatin基因真核重组质粒的构建及鉴定.docVIP

反义cortactin基因真核重组质粒的构建及鉴定 ：许朱定，宋振顺，安家泽，李韧，张福琴，窦科 峰 关键词】，基因表达 AIM：Tocon AIM：Tocon struetarecombinant Construction frecombinanteukary orofhumananti sense iatedprotEingene Abstract 】 eukaryoticexpressi ningfunctionalregi corticalactinassoc ortactin),soastopr rtherstudyontheimp patocellularcarcin oma(HCC)metastasis asextractedbyusing andidentificationo oticexpressionvect corticalactinassoc on/cortactincontai onofhumanantisense iatedprotEingene(c ovideatoolforthefu actofcortactininhe .METHODS：TotalRNAw Trizolonestepmetho dfromcel1lineMHCC9 dfromcel1lineMHCC9 usingreversetransc hainreaction(RTPC