海州常山授粉受精及杂交育种.PDFVIP

安徽农业大学学报, 2018, 45(5): 883-888 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x001 网络出版时间：2018/11/2 11:10:00 [URL] /kcms/detail/34.1162.S1113.002.html 海州常山授粉受精及杂交育种 1 1 1 1 2 杨秀莲 ，张莹婷 ，何 岭 ，王良桂 ，赵 荣 (1. 南京林业大学风景园林学院，南京 210037 ；2. 南京林业大学生物与环境学院，南京 210037) 摘 要：为推进海州常山杂交育种进程，以 11 个地理种源为试材，采用荧光显微镜及石蜡切片法观察授粉受 精过程，亦对21 个种内杂交组合进行人工杂交授粉，统计座果率探究杂交亲和性。结果表明：授粉0.5 h，母本柱 头上花粉未萌发；授粉1 h 有少量花粉萌发；授粉2 h 花粉管生长至花柱基部；授粉4 h ，花粉大量萌发，花粉管伸 入胚珠；授粉8 h，大量花粉管达花柱基部，胼胝质增多；花粉管伸入胚囊发生在授粉后1 d，精细胞与卵细胞、极 核融合发生授粉后2 d ，授粉后3 d 合子开始原胚生长。杂交组合座果率分布范围0.00%～73.33%，母本座果率分 布为0.00%～52.94%，父本座果率在0.00%～46.08% ，通过正反交可提高座果率0.00%～363.00% 。 关键词：海州常山；授粉受精；杂交育种 中图分类号：S685.99 文献标识码：A 文章编号：1672352X (2018)05088306 Pollination, fertilization and cross breeding of Clerodendrum trichotomum 1 1 1 1 2 YANG Xiulian , ZHANG Yingting , HE Ling , WANG Lianggui , ZHAO Rong (1. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037; 2. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037) Abstract: Using the method of fluorescent microscope and paraffin section, this study was conducted on pollen tube growth and double fertilization process in Clerodendrum trichotomum for speeding up process of Clerodendrum trichotomum cross-breading. In addition, 11 geographic provenances of Clerodendrum trichoto- mum were also used as parents in 21 reciprocal cross combinations, and cross compatibility among these geo- graphic provenance