东北连翘鲜花精油的GC-MS分析-国家级大学生创新创业训练计划平台.PDF

东北连翘鲜花精油的GC-MS 分析 *1 安旆其，王海英 ，曹旭东，杜思琪，张璐 (东北林业大学 林学院，黑龙江 哈尔滨150040） （生物质化学工程2015.11.30） 王海英 副教授 摘要：采用同时蒸馏萃取（SDE）法提取东北连翘鲜花精油，气相色谱-质谱法（GC-MS）分析精油的化学 组分。考察不同溶剂对结果的影响，结果表明SDE 石油醚萃取法得到的东北连翘鲜花精油得率为0.69%， 鉴定28 种成分，GC 含量最高的化合物为二十五烷(28.65%)，其次是Z-9-二十三烯(27.82%)。SDE 正己烷 萃取法得到的东北连翘鲜花精油得率为 0.81%，鉴定 44 种成分，GC 含量最高的化合物为 Z-9-二十三烯 (47.16%)，Z-9-二十三烯是家蝇性引诱剂，其他香料化合物如肉豆蔻醛(0.97%)、石竹烯(0.05%)等。 关键词：东北连翘；鲜花精油；同时蒸馏萃取；石油醚；正己烷；GC-MS 分析 中图分类号：TQ35;TQ651;Q946.85 文献标识码：A 文章编号：1673-5854(2015)06-0000-00 GC-MS Analysis of Essential Oil of Forsythia mandschurica Uyeki Fresh Flowers * AN Pei-qi,WANG Hai-ying ,CAO Xu-dong,DU Si-qi,ZHANG Lu (College of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China) Abstract ：Essential oils of Forsythia mandschurica Uyeki fresh flowers were extracted by simultaneous distillation extraction(SDE) method.Chemical composition of essential oils were analysized by gas chromatography-mass spectrometry(GC-MS) method. The effects of different solvents on the results were studied. The results showed that the yield of essential oil of F. mandschurica Uyeki fresh flower extracted by SDE light petroleum method was 0.69%, 28 components were identified, the highest GC content compound was pentacosane(28.65%), abd the second was Z-9-tricosene(27.82%). The yield of essential oil of F. mandschurica Uyeki fresh flower extracted by SDE hexane method was 0.81%, 44 components were identified, the highest GC content compounds was Z-9-tricosene(47.16%), the Z-9-tricosene was housefly sex attractant, and other aromatic compounds were myristic aldehyde(0.97%), caryophyllene(0.05%), etc. Key words: Forsythia mandschurica Uyeki;fresh