大豆异黄酮对糖尿病大鼠心肌肌浆网钙离子调控蛋白和细胞膜KATP通道的作用-病理学与病理生理学专业论文.docx

明显减轻。4. RT-PCR检测：与NC组比，DC组大鼠心肌SERCA、RyR2、IP3R2 mRNA 表达显著降低，但PLB mRNA表达显著增高（P＜0.01）。与DC组比，ST组、 NT组大鼠心肌SERCA、RyR2、IP3R2 mRNA表达显著增高，而PLB mRNA表达显 著降低（P＜0.01）。ST、NT组KATP的SUR2和Kir6.2表达水平与DC组及NC相比 无显著差异(P＞0.05)。5. 免疫组化Elivision法检测SERCA2a蛋白的变化：DC组 SERCA2a蛋白表达明显低于正常组，ST、NT组SERCA2a蛋白表达高于DC组（P ＜0.01）。 结论：大豆异黄酮（SI）可减轻糖尿病大鼠心肌结构损伤，改善左心室功能，对 糖尿病大鼠心肌损伤有保护作用。其作用机制可能与 SI 上调钙调控蛋白 SERCA、 RyR2、IP3R2 mRNA 和 SERCA2a 蛋白的表达，下调 PLB mRNA 的表达有关，而 对糖尿病大鼠心肌组织 KATP 通道 mRNA 的表达无显著影响。 关键词：大豆异黄酮； 糖尿病； 心肌； 肌浆网； 钙调控蛋白； 基因表达 Effect of soy isoflavones on myocardial sarcoplasmic reticulum calcium handling proteins and myocardial sarcolemmal ATP-sensitive potassium channel in diabetic rats Abstract Objective: To study the influence of soy isoflavones(SI) on mRNA and/or protein expression of myocardial sarcoplasmic reticulum calcium handling proteins and myocardial sarcolemmal ATP-sensitive potassium channels (sarcol KATP) in diabetic rats. To investigate the mechanism of protection effect of SI on diabetic cardiomyopathy. Methods: Sixty male Sprague-Dawley rats were randomly divided into four groups: normal control group (NC), diabetic control group (DC), soy isoflavones treatment group (ST) and nilestrioli treatment group (NT). Rats were given streptozotocin (STZ) 55 mg·kg-1 intraperitoneal injection except NC group. After 6 weeks, SI 120 mg·kg-1·d-1 was given in ST group, nilestrioli (NI) 0.2 mg·kg-1 was given in NT group twice a week, and the same CMC-Na were given in NC and DC groups . Volumes in all groups were 2 ml·kg-1·d-1. Drugs were all intragastric administered using stomach tubes. LVSP (left ventricular systolic pressure), LVEDP (left ventricular end diastole pressure), Mean, ±dp/dtmax of rats were recorded. The gene expressions of calcium handling proteins sarco/endoplasmic reticulum Ca2+-ATPase(SERCA), ryanodine receptor type 2 (RyR2), 1,4,5-trisphosphate inositor type 2 (IP3R2), SUR2 and Kir6.2 of KATP were detected by RT-PCR. The protein expression of SERCA2a were detected expre