有机溶剂中脂肪酶催化辛酸辛酯的合成-食品科学.PDF

44 2010, Vol. 31, No. 15 食品科学 ※基础研究 1 2, 张凤秀 ，张光先 * (1.西南大学化学化工学院，重庆 400715 ；2.西南大学纺织服装学院，重庆 400715) ：以猪胰腺脂肪酶为催化剂合成辛酸辛酯。研究有机溶剂、反应温度、加水量、加酶量、反应时间、底 物浓度及底物物质的量比 7 个因素对酶促酯化反应的影响，优化得到最佳合成条件。结果表明：最佳反应温度 45 ℃、正己烷有机溶剂、加水量29 μL 、酶质量浓度2.314g/L 、反应时间24h 、辛酸与辛醇物质的量比为 1:1.2～ 1 13 1:1.5、辛酸浓度为0.23～0.33mol/L 。在优化条件下，辛酸的转化率达到98.12% 以上，并通过IR 、H NMR 、 C NMR 谱表征了产物结构。酶催化法能在温和的反应条件下合成高产率的酯。 ：脂肪酶；合成；辛酸辛酯；有机溶剂；酯化反应 Enzymatic Synthesis of Caprylic Caprylate in an Organic Solvent 1 2, ZHANG Feng-xiu ，ZHANG Guang-xian * (1. College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China； 2. College of Textile and Garment, Southwest University, Chongqing 400715, China) Abstract ：The synthesis of caprylic caprylate catalyzed by lipase from porcine pancreas in an organic solvent was carried out to explore the effects of organic solvent type, reaction temperature, amount of added water, reaction time, lipase amount, substrate concentration and caprylic acid-to-caprylic alcohol ratio on caprylic acid conversion. The optimum organic solvent for caprylic caprylate synthesis was n-hexane. Reaction temperature of 45 ℃, amount of added water of 29 μL, caprylic acid-to- caprylic alcohol ratio of 1:1.2 －1:1.5, caprylic acid concentration of 0.23－0.33 mol/L, lipase concentration of 2.314 g/L were found optimum. Under these optimized conditions, the conversion ratio of caprylic acid was 98.12%. In addition, the structure