分子对接优选GGPPS模块与酿酒酵母底盘适配性研究-制药工程专业论文.docx

The fitness of chassis and heterologous modules is the key point in heterologous production of target compounds. In this study, rational design of taxadiene biosynthetic yeast was carried out based on predicting the structures and catalytic capabilities of six key enzymes-GGPPS from different sources using enzyme-substrate docking method. The computational identification method can be useful for our rational design in application of synthetic biology. While the metabolomics analysis can give us more informations on the interactions of chassis and heterologous modules. So that it can be developed into an universal method in the research of compounds heterologous production. The new strategies or methods applied in this study can provide more informations on the further research of fitness between chassis and heterologous modules. KEY WORDS：Taxadiene, Saccharomyces cerevisiae, molecular docking, fitness, heterologous modules, metabolomics 目 录 HYPERLINK \l _bookmark0 前 言 HYPERLINK \l _bookmark0 1 HYPERLINK \l _bookmark1 第一章 文献综述 HYPERLINK \l _bookmark1 2 HYPERLINK \l _bookmark1 1.1 概述 HYPERLINK \l _bookmark1 2 HYPERLINK \l _bookmark2 1.2 合成生物学技术在酵母生产萜类化合物中的应用 HYPERLINK \l _bookmark2 3 HYPERLINK \l _bookmark2 1.2.1 酿酒酵母作为底盘细胞的优势与需要解决的问题 HYPERLINK \l _bookmark2 3 HYPERLINK \l _bookmark3 1.2.2 调控基因表达水平的方法 HYPERLINK \l _bookmark3 5 HYPERLINK \l _bookmark3 基因表达水平与拷贝数 HYPERLINK \l _bookmark3 5 HYPERLINK \l _bookmark4 不同强度与类型的启动子 HYPERLINK \l _bookmark4 7 HYPERLINK \l _bookmark5 基于RNA水平的调控 HYPERLINK \l _bookmark5 9 HYPERLINK \l _bookmark6 基于蛋白水平的调控 HYPERLINK \l _bookmark6 10 HYPERLINK \l _bookmark7 1.2.3 调控相关酶空间位置的策略 HYPERLINK \l _bookmark7 11 HYPERLINK \l _bookmark7 蛋白融合策略 HYPERLINK \l _bookmark7 11 HYPERLINK \l _bookmark8 酶固定于统一架构 HYPERLINK \l _bookmark8 12 HYPERLINK \l _bookmark9 酶定位于内源细胞器 HYPERLINK \l _bookmark9 13 HYPERLINK \l _bookmark10 1.2.4 酵母中生产萜类化合物的研究进展 HYPERLINK \l _bookmark10 14 HYPERLINK \l _bookmark11 类胡萝卜素在酵母中的生产 HYPERL