单性木兰的遗传多样性分析-生物化学与分子生物学专业论文.docx

PAGE PAGE IV Study on the genetic diversity of Kmeria septentrionalis Dandy Speciality: biochemistry and molecule biology Subject: molecule phylogenesis Master Candidate:Liyan Zeng Grade:2004 Supervisor: Prof. Shaoqing Tang Abstract Kmeria septentrionalis Dandy, an endemic genus to China, belongs to the family Magnoliaceae, which are listed among Grade 1 National Protected Rare and Endangered Plants. In China, Kmeria septentrionalis Dandy mainly grows wild in the southwestern region (Mulun Huangjiang and Luocheng Guangxi Province, Maolao libo Guizhou Province，Maguan Yunnan Province ) .Magnoliaceae is one of the most primative angiosperm , Kmeria septentrionalis Dandy is triecious which is a especial species in the evolution of Magnoliaceae, it has very high value in scientific research. At the same time, it grows quickly as good timber so it is valuable and significance in forestry and research.。 According to the distribution of Kmeria septentrionalis Dandy, thirteen populations are sampled (eight populations from Mulun, Huangjiang,Guangxi, two populations from Luocheng, Guangxi, three populations from Maolan , Libo, Guizhou). Genetic diversity and genetic structure of Kmeria septentrionalis Dandy were investigated by using amplified fragment length polymorphism (AFLP) markers. An understanding of both genetic diversity and genetic structure of Kmeria septentrionalis Dandy provides insight for the conservation and management of this endangered species . The main results were as follows: Amplified results: A total of 6 AFLP primer combinations were employed on 260 individuals of Kmeria septentrionalis Dandy from 96 primer combinations. These primers generated a total of 317 bands, of which 157 were polymorphic. Genetic diversity At species level, the percentage of polymorphic loci (P) was 49.53%, Nei’s gene diversity (He) was 0.1289, and Shannon’s information index (I) was 0.2004, respectively. At population level, the estimates of P =31.13%(27.76%?35.02%)