小牛脾提取物注射液对小鼠的抗疲劳活性研究.PDFVIP

小牛脾提取物注射液对小鼠的抗疲劳活性研究   刘鑫，卢文倩，蔡广胜，蒋天悦，杨静，刘洋，权宇彤(吉林大学生命科学学院，长春 130012) 摘要：目的 研究小牛脾提取物注射液(Calf Spleen Extractive injection ，CSEI)对小鼠的抗疲劳功效，并探讨其初步机制。 方法 以胸腺法新(T1)作为对照药，进行小鼠运动耐力试验，检测运动后小鼠血清中疲劳和氧化应激相关生化指标以及 激素水平。结果 腹腔给药21 d 后，CSEI 能够提高小鼠的跑步和负重游泳时间；降低血浆、肝脏和肌肉中的乳酸含量， 降低肝脏和肌肉中的乳酸脱氢酶含量，提高肝糖原和肌糖原含量；另外，CSEI 能够提高小鼠血浆、肝脏、肌肉中的超氧 化物歧化酶和谷胱甘肽过氧化物酶的水平，降低肝脏和肌肉中的活性氧簇水平，调节血浆中激素睾酮和皮质醇的水平。 结论 CSEI 的抗疲劳活性可能是通过抗氧化和调节体内激素水平来实现的。 关键词：小牛脾提取物注射液；抗疲劳；抗氧化；激素 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2018)11-1613-05 DOI: 10.13748/ki.issn1007-7693.2018.11.004 引用本文：刘鑫, 卢文倩, 蔡广胜, 等. 小牛脾提取物注射液对小鼠的抗疲劳活性研究[J]. 中国现代应用药学, 2018, 35(11): 1613-1617. Study on the Antifatigue Effect of Calf Spleen Extractive Injection on Mice * LIU Xin, LU Wenqian, CAI Guangsheng, JIANG Tianyue, YANG Jing, LIU Yang, QUAN Yutong (School of Life Sciences, Jilin University, Changchun 130012, China) ABSTRACT: OBJECTIVE To evaluate the antifatigue effect of Calf Spleen Extractive injection(CSEI) on mice and to explore its preliminary mechanism. METHODS Exercise tolerance test, fatigue and oxidation related biochemical indices and plasma hormone level were detected with Thymosin1(T1) served as control drug. RESULTS After 21 d of intraperitoneal administration, CSEI improved the running and loading swimming time of mice significantly. CSEI could reduced the LD(in plasma, liver and muscle) and LDH(in liver and muscle) levels simultaneously, and increased the liver glycogen and muscle glycogen. Meanwhile, CSEI improved the superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) levels in plasma, liver and muscle, decreased reactive oxygen species(ROS) level both in