激光散射法研究蛋白质与氨比西林钠盐的相互作用.PDF

B 2006, 36 (1): 71~75 71 * (, 730000) 25 0.1 , 0.085 mol ·kg− 1, pH 7.4, 5.8, 4.9 . , pH 7.4, 5.8, 4.9 , : 210000, 418000, 448000 g ·mol− 1; pH. pH . , / , pH. (HSA) 1 pH [9]. / . , HSA , [1~5]. , . , . , pH (HSA), , . [1~4, 6], 585 2 , ( 0.15 − 1 [7] [8] 2.1 mol ·L NaCl 4.9) . Krauss (Brookhaven, America), 4 W . ; (Millipore, America); CS501 : 2005-04-02; : 2005-08-19 * , E-mail: huzd@ SCIENCE IN CHINA Ser. B Chemistry 72 B 36 (); PHS-10A ( c (g ·cm−3) , Rθ (cm− 1) θ ); RF-5301C Rayleigh ; Mw (g ·mol− 1) ; A2 (Shimadzu, Japan); . (cm3 ·g−2 ·mol)Virial; Rg (nm) HSA Sigma; (6-(-)- ; q = (4πns/λ)sin(θ/2), ; KZ )(pKa1=2.65, pKa2=7.24)Sino (mol ·cm2 ·g−2), . , : () . 0.2 mol ·L− 1 K ⎡2