干预肥大心肌细胞代谢途径的抗凋亡效应研究-内科学(心血管病)专业论文.docx

第三军医大学硕士学位论文 第三军医大学硕士学位论文 英文缩写一览表 英文缩写 EDTA 英文全称 Ethylene diaminetetraacetic acid 中文词义 乙二胺四乙酸 PBS Phosphate buffered saline 磷酸盐缓冲液 FBS [3H]-Leu Fetal bovine serum [3H]-Leucine 胎牛血清 [3H]-亮氨酸 CoASH Coenzyme A 辅酶 A AST Aspartic acid transferase 门冬氨酸转移酶 NAD Nicotinamide-adenine dinucleotide 烟酰胺腺嘌呤二核苷酸 PDH Pyruvate dehydrogenase 丙酮酸脱氢酶 PDHa Active PDH 活性丙酮酸脱氢酶 PDHt Total PDH 总丙酮酸脱氢酶 CPT-1 Carnitine palmitoyltransferase 1 肉碱脂酰转移酶 1 TMZ Trimetazidine 曲美他嗪 DCA Dichloroacetate 二氯乙酸 NE Norepinephrine 去甲肾上腺素 Angl Angiotentionl 血管紧张素l TNF Tumor necrosis factor 肿瘤坏死因子 EGTA Egtazic acid 四羧酸酯酸 GLUT-1 Glucose transporter 1 葡萄糖转移因子 1 GLUT-2 Glucose transporter 2 葡萄糖转移因子 2 PFK Phosphfructokine 磷酸果糖激酶 μg Microgram 微克 L Litre 升 g Gram 克 mg Milligram 毫克 min Minute 分钟 h Hour 小时 r Rotation 转 1 2 2 Antiapoptotic effect of metabolism pathway intervention in hypertrophied cardiomyocytes Abstract Many studies have shown that apoptosis gets involved in the mechanisms that myocardium hypertrophy transfers to heart failure, and plays a important role in pathologic injury caused by ischemia-reperfusion. Recently, apoptotic biochemical process and signal transduction has been clarified, that is to say ,apoptotic pathway mediated either by mitochondria or death receptors activate caspase cascade to induce cell apoptosis. The mechanism that apoptosis regulated by mitochondria is widely supported. Myocardium is a kind of tissue with active energy metabolism, and containing abundant mitochondria. Mitochondial chief function is energy metabolism, which plays a vital role in maintenace of cellular physiological functions. Recent studies indicated that mitochondria were tightly related with cell apoptosis, and possessed function of regulating apoptosis. Abnormalities of mintochondrial metabolism includes alteration of energy metabolism pathways and injury of electron transport chain, but their relationship with apoptosis is undiscovered. When hypertrophied, myocardium energy metabolic model markedly cha