肺原位腺癌、微浸润腺癌及浸润性腺癌中淋巴管密度的变化.PDFVIP

J South Med Univ, 2018, 38(11): 1349-1353 doi 10.12122/j.issn. 1673-4254.2018.11.12 ·1349 · 肺原位肺原位腺癌腺癌、、微浸润腺癌及浸润性腺癌中淋巴管密度的变化及微浸润腺癌及浸润性腺癌中淋巴管密度的变化及 其调控其调控因子因子 何 萍，顾 霞，曾 欣，郑咏玫，林晓东 广州医科大学附属第一医院病理科，广东 广州 510120 摘要：目的分析肺原位腺癌、微浸润腺癌及浸润性腺癌中肿瘤淋巴管密度的变化及其可能的调控因子。方法 收集具有完整临 床病理资料的肺腺癌病例301 例并分为原位腺癌（n=28 ，9.3% ）、微浸润腺癌（n=86 ，28.6% ）及浸润性腺癌（n= 187，62.1% ）3 组。 应用D2-40 免疫组化标记计数各组肺腺癌中淋巴管密度，同时检测MT 1-MMP、VEGF-C 蛋白表达水平。分析肺腺癌3 种病理 类型病例中淋巴管密度的变化差异及其与MT 1-MMP、VEGF-C 蛋白表达、临床病理因子（特征）的关系。结果 淋巴管密度在分 析肺原位腺癌、微浸润腺癌及浸润性腺癌组中的变化差异具有统计学意义（P=0.000）；淋巴管密度与VEGF-C 蛋白表达水平具 有相关性（r=0.917, P=0.009）。淋巴管密度与肿瘤大小（r=0.686, P=0.017）、淋巴结转移（r=0.739, P=0.000）及临床分期（r= 0.874, P=0.012）具有相关性。结论 肿瘤淋巴管生成可能与肺腺癌进展过程和临床病理因子（特征）密切相关，VEGF-C 可能是 促进肿瘤淋巴管生成的调控因子。 关键词：肺腺癌；淋巴管密度；VEGF-C ；MT 1-MMP Changes of lymphatic vessel density in lung adenocarcinoma in situ, minimally invasive adenocarcinoma, and invasive adenocarcinoma and the regulatory factors HE Ping, GU Xia, ZENG Xin, ZHENG Yongmei, LIN Xiaodong Department of Pathology, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China Abstract: Objective To analyze the changes in tumor lymphatic vessel density (LVD) in patients with lung adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and invasive adenocarcinoma (IA) and explore the regulatory factors of LVD. Methods Complete clinicopathological data were collected form a total of 301 patients with lung adenocarcinoma, including 28 (9.3% ) with AIS, 86 (28.6% ) with MIA, and 187 (62.1% ) with IA. The LVD of all the adenocarcinomas were calculated after D2-40 immunohistochemical staining, and MT1-MMP and VEGF-C expression levels were also evaluated. The differences in LVD among the groups and the correlations of tumor LVD with the expressions of MT1-MMP and VEGF-C and the clinicopathological factors were analyzed. Results The LVD differed signifi