PCV2感染猪皮肤源树突状细胞内源性抗原.PDFVIP

中国农学通报 2011,27(29):33-37 Chinese Agricultural Science Bulletin PCV2感染猪皮肤源树突状细胞内源性抗原 加工提呈相关分子转录水平的变化 李建东，李焕荣，聂晓华，遇 奇，崔德凤 （北京农学院动物科学技术学院，北京102206） 摘 要：用猪圆环病毒2 型(PCV2)感染40 日龄健康长白仔猪，于感染后3、7、14、21、35 天宰杀，收集皮肤 源树突状细胞（DC ）。利用实时荧光定量PCR 技术对感染仔猪皮肤源DC 内源性抗原加工提呈相关分 子（LMP7 ，UBP ，MHC-I ，calreticulin ）mRNA 水平的变化进行定量分析。结果显示，LMP7 转录水平在感 染后3 天显著下调（P0.05 ），UBP 转录水平始终上调，其中在21 天和35 天显著上调（P0.05 ），MHC-I 转 录水平在7 天显著下调（P0.05 ），calreticulin 转录水平虽有所上调，但差异不明显。以上结果表明， PCV2 在感染早期可抑制猪皮肤源DC 内源性抗原加工提呈能力。 关键词：猪圆环病毒2 型；皮肤源树突状细胞；内源性抗原加工提呈；实时荧光定量PCR 中图分类号：S858.285.3 文献标志码：A 论文编号：2011-0836 ChangesoftheTranscriptionLevelsofMoleculesAssociatedwithEndogenousAntigenProcessing andPresentationofPorcineSkin-derivedDendriticCellsInfectedwithPCV2invivo LiJiandong,LiHuanrong,NieXiaohua,YuQi,CuiDefeng (College of Animal Science and Technology, Beijing University of Agriculture,Beijing102206) Abstract: Healthy Large White piglets of forty-day-old were euthanized on 3, 7, 14, 21 and 35 days post-infected with porcine circovirus type 2 (PCV2) and their skin-derived dendritic cells (DCs) were collectedforanalyzingthetranscriptionlevelsofmolecules(LMP7,UBP,MHC-I,calreticulin)associatedwith endogenous antigen processing and presentation by real-time fluorescent quantitative PCR (real-time FQ-PCR).TheresultsshowedthatthetranscriptionlevelsofLMP7mRNAsweredecreasedsignificantlyon3 days post inoculation (DPI) (P0.05). UBP and calreticulin mRNAs were consistently higher during the experiment,onlywithsignificantdifferenceson21and35DPI(P0.05)forUBP.AndMHC-Iweredecreased significantlyon 7 DPI(P0.05).Alloftheaboveresultsindicatedareductionintheabilityofendogenous antigenprocessingandpresentationofporci