油菜菊花和荷花蜂花粉中磷脂的色谱分析-食品科学.PDF

※分析检测 食品科学 2011, Vol. 32, No. 18 185 1,2 1 1, 2 1,2 1,2 1,2 何 伟 ，徐 响 ，孙丽萍 * ，庞 杰 ，黄 兰 ，穆雪峰 ，沈新锋 (1. 中国农业科学院蜜蜂研究所，北京 100093 ；2.福建农林大学食品科学学院，福建 福州 350002) 以油菜、菊花、荷花蜂花粉为原料，采用薄层层析法(thin layer chromatography ，TLC)法分离纯化蜂花 粉中的磷脂，并用高效液相色谱法(high-performance liquid chromatography ，HPLC)法测定磷脂酰肌醇(PI) 、磷脂 酰丝氨酸(PS)、脑磷脂(PE)、卵磷脂(PC)及溶血卵磷脂(LPC) 的含量。结果表明：3 种蜂花粉中总磷脂含量为 1.19～ 3.98g/100g，3 种花粉存在极显著差异(P ＜0.01)；PC 是蜂花粉磷脂的主要成分，占总磷脂的 34.30%～59.69% ；在 油菜蜂花粉中检测到 PI 、PS 、PE 、PC 、LPC ，菊花蜂花粉未测出PI ，荷花蜂花粉未测出PI 、LPC 。结论： 油菜蜂花粉中磷脂种类最丰富、总含量最高，是花粉磷脂的较好来源。 蜂花粉；高效液相色谱法(HPLC) ；磷脂；含量 Chromatographic Analysis of Phospholipids in Rape, Chrysanthemum, and Lotus Bee Pollens 1,2 1 1, 2 1,2 1,2 1,2 HE Wei ，XU Xiang ，SUN Li-ping *，PANG Jie ，HUANG Lan ，MU Xue-feng ，SHEN Xin-feng (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China； 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstract ：The phospholipids in rape, chrysanthemum, and lotus bee pollens were separated and purified by TLC, and were quantitatively determined by HPLC. The results indicated that the total phospholipid contents in three bee pollens were from 1.19 to 3.98 g/100 g with a significant difference (P ＜0.01). Phosphatidylchuline (PC) was the predominant phospholipid in bee pollen, representing 34.30% －59.69% of total phospholipids. Phosphatidylinositol (PI), phosphatidylserine (PS), phosphati- dyl ethanolamine (PE), phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) were detected in rape bee pollen, but no PI in chrysanthemu