河流七氯降解菌的筛选和降解影响因素研究-环境工程专业论文.docxVIP

万方数据 万方数据 Separation of river heptachlor degrading bacteria and its degradation influence factor research A Dissertation Submitted for the Degree of professional Master Candidate：Zhang Xiaolin Supervisor：Prof. Qiu Liping Chang’an University, Xi’an, China 摘 要 本实验对取自渭河底泥天然厌氧微生物进行培养驯化、分离纯化，从中分离出三种 有降解能力的菌株，在相同条件下降解七氯，测定、比较降解率，筛选出能够高效降解 七氯的优势降解菌株，并分别研究了不同因素对微生物降解七氯的影响，得出了该优势 菌株降解七氯的最适环境条件，为七氯的微生物降解提供了一定的方法参考和理论依 据。 本实验得到以下结论： 1、实验以渭河天然微生物为菌源，在温度为 15℃，pH 值为 7 的条件下密封避光培 养，在驯化过程中，通过不断提高培养液中七氯的浓度，筛选出了能够在厌氧环境下降 解七氯的微生物菌群，降解菌最终的驯化浓度为 0.2mg/L； 2、将驯化得到的微生物菌群进行分离纯化，得到三种不同的菌株（Z1、Z2、Z3）， 在温度为 15℃，pH 值为 7，七氯初始浓度为 0.2mg/L 的厌氧条件下降解十天后，Z2 菌 株的降解效果最好，为本实验筛选出的优势菌株； 3、实验研究了不同温度、不同 pH、不同外加碳源、不同七氯初始浓度、不同微生 物加入量以及不同菌种混合对优势菌株降解七氯的影响，得到了 Z2 菌株降解七氯的最 适条件为：温度为 25℃，pH 值为 8.0，加入葡萄糖为外加碳源，七氯初始浓度为 0.2mg/L， 优势菌种储备液（1.32 x108 个/毫升）加入量为 100 毫升，Z2 单菌株进行降解。 以上实验结果表明，从渭河成熟底泥中能够筛选出七氯降解菌，筛选出的优势降解 菌 Z2，对七氯有较好的降解效果。 关键词：七氯；渭河；微生物降解；筛选；影响 I Abstract In this study, the natural anaerobic microorganisms taken from WeiHe River sediment is cultured and acclimated, then isolated and purified. Three strain which has the ability of degradation is isolated from the microorganisms. After that, we degrade heptachlor in the same conditions, measure and compare the rate of degradation. And then screen out degrading bacteria that can be able to degrade heptachlor efficiently. Besides that, study the effects of different factors on the microbial degradation of heptachlor, obtain optimum environmental conditions of predominant strains for degrading heptachlor, providing some reference methods and theoretical basis for microbial degradation of heptachlor. In this study，we can draw the following conclusions: The bacteria source of the experiment is from WeiHe natural Microorganism. Culture microorganism in sealed dark conditions at the temperature of 15 ℃ and pH value of 7. In the domestication process, through continuous improvement heptachlor’s concentration of culture medium, screened out microbial flora which can deg