油茶生长发育过程中脂肪酸成分的测定分析.PDFVIP

中国农学通报 2012,28(13):76-80 Chinese Agricultural Science Bulletin 油茶生长发育过程中脂肪酸成分的测定分析 1 1 1 2 王小艺 ，曹一博 ，张凌云 ，陈永忠 1 2 （北京林业大学森林培育与保护教育部重点实验室，北京100083；湖南省林科院，长沙410004 ） 摘 要：为了研究油茶生长发育过程中脂肪含量及脂肪酸成分的变化，分析测定2 个油茶品种在不同生 长时期油茶籽中的脂肪酸成分。采用索氏提取法提取油茶籽中的脂溶性成分，经甲酯化处理后用气象 色谱分离和鉴定，运用面积归一法确定各成分的相对百分含量。结果表明，在整个发育过程中油脂形成 有2 个高峰，分别在9 月上旬和10 月上旬；种子种脂肪酸的主要组成是不饱和脂肪酸，油酸含量最高，其 次是亚油酸和油酸，饱和脂肪酸以棕榈酸和硬脂酸为主，其中油酸呈明显上升趋势，棕榈酸和亚油酸呈 明显下降趋势，硬脂酸呈小幅上升趋势，亚麻酸呈小幅下降趋势。 关键词：油茶籽；脂肪酸；索氏提取法；气象色谱；不饱和脂肪酸 中图分类号：S727.3 文献标志码：A 论文编号：2012-0460 AnalysisoftheFattyAcidsCompositionsofCamelliainDifferentGrowthStages 1 1 1 2 Wang Xiaoyi , Cao Yibo , Zhang Lingyun , Chen Yongzhong 1 ( KeyLaboratoryforSilvicultureandConservationofEducationMinistry,BeijingForestryUniversity, Beijing 100083; 2HunanForestryandSciencesInstitute, Changsha 410004) Abstract: The aim was to research the changes of fat content and fatty acid composition in the growth and development of Camellia. The fatty acids compositions of two oil-tea species in different growth stages of camellia seeds were analyzed and determined in this present. The fat-soluble ingredients of camellia were extracted using the soxhlet extractor. Then the fatty acids compositions were separated and analyzed after methyl processing meteorological by gas chromatograph, using area normalization method to determine the relative contents of each ingredient. The results indic