《外源SA对持续低温胁迫藜豆幼苗的生长及生理特性的影响》-毕业设计论文（学术).docVIP

PAGE 外源SA对持续低温胁迫藜豆幼苗的生长及生理特性的影响 学生：×××，江汉大学生命科学学院 指导老师：×××，江汉大学生命科学学院 摘要 [目的]探讨外源SA在低温胁迫下对藜豆幼苗抗冷性的影响以及确定提高幼苗抗寒性适宜浓度。[方法]分别用5℃和10℃低温胁迫2叶一心的藜豆幼苗，观察植株生长的变化以及在10℃低温胁迫下，用0、1、2、3、4mmol/L的水杨酸喷藜豆幼苗叶片，预处理3天，观察对株高、鲜重等生长指标和对蛋白质、丙二醛、脯氨酸、膜透性等生理指标的影响。[结果]（1）藜豆植株能忍受10℃的低温，而5℃的低温会使植株死亡；（2）10℃低温下，4mmol/L的SA抑制幼苗的生长，而1、2、3mmol/L的SA对植株的株高和鲜重没有显著影响；（3）1-4mmol/L的SA预处理能提高藜豆幼苗叶片中的蛋白质含量，2-4mmol/L的SA能使游离脯氨酸含量增加；（4）1-4mmol/L的SA对反应膜稳定性的指标丙二醛含量和膜透性影响不大。[结论]（1）5℃的低温使藜豆致死；（2）2-3mmol/L的外源SA可通过提高体内的蛋白质和脯氨酸含量来增强藜豆幼苗的抗冷性。 关键词 藜豆；低温胁迫；外源SA；抗冷性 Effects of exogenous salicylic acid on the growth and physiological characteristics of velvetbean seedlings under continual low temperature stress Student: Cui Yawei（college of life science, Jianghan university, wuhan 430056) Tutor: Zhang Fengyin（college of life science, Jianghan university, wuhan 430056) Abstract Objective To explore the effects of exogenous salicylic acid（SA）under low temperature stress on cold tolerance of velvet seedlings and to determine the suitable treatment concentrations. Materials and methods Respectively 5℃ and 10℃ low temperature stress as one of two leaves to observe changes in plant growth.In this paper, under 10℃ low temperature stress on leaves applicated 0,1,2,3,4 mmol/L exogenous salicylic acid pretreated 3 days, and observe the morphological changes, measuring its plant height, fresh weight and protein content, MDA content, proline content, Relative electrolyte leakage and other physiological indicators, and through these data to discuss salicylic acid on cold resistance of seedling. Results 1)The seedlings can endure 10℃, while 5℃ the seedlings will die; 2) Under 10℃,4mmol/L of SA inhibited the growth of seedlings, and1,2,3mmol/L of SA pre-treated on height and weight had no significant effect; 3)1-4mmol/L of SA pretreated can improve the velvetbean seedlings protein content in leaves,2-4mmol/L of SA pretreated can increase free proline content; 4)1-4mmol/L of SA pretreated on MDA and Relative electrolyte leakage h