利用衍生化试剂DTNB测定枸杞中谷胱甘肽-食品科学.PDFVIP

250 2011, Vol. 32, No. 06 食品科学 ※分析检测 朱亚玲，张小勇，崔胜云 * (延边大学 长白山生物资源与功能分子教育部重点实验室， 吉林 延吉 133002) 利用 5,5 ＇- 二硫硝基苯甲酸(DTNB)衍生化试剂和植物材料同步破壁提取的样品处理方法，采用电喷雾质谱 法和分光光度法对枸杞中还原型谷胱甘肽(GSH)进行定性、定量分析。枸杞样品提取过程中 GSH 受破壁后胞外化学 环境的影响导致氧化变性。利用 5,5 ＇- 二硫硝基苯甲酸(DTNB)衍生化试剂同步提取，因生成稳定的 GSH 的衍生化 产物避免了 GSH 氧化变性导致的 GSH 分析信息丢失。用 UV-Vis 分光光度法测定 GSH 含量，发现枸杞中含有丰 富的GSH 。标准加入法测定枸杞干品中GSH ，其含量范围达(6.56 ±0.54)mg/g ，是目前所测植物样品中GSH 含量 较高的物种。本研究对药用植物胞内活性物质的准确分析提供方法学的尝试，并通过测定发现枸杞中富含 GSH 。 谷胱甘肽；5,5 ＇- 二硫硝基苯甲酸；枸杞；分光光度法；质谱分析法；同步衍生化 Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent ZHU Ya-ling ，ZHANG Xiao-yong ，CUI Sheng-yun* (Key Laboratory of Natural Resources of Changbai Mountain Functional Molecules，Ministry of Education, Yanbian University, Yanji 133002, China) Abstract ：The content of reduced glutathione (GSH) in Lycium barbarum L fruits was determined by mass spectroscopy and UV-visible spectroscopy using 5,5 ＇-dithio-bis-nitrobenzoic acid (DTNB) as the simultaneous derivatizing agent. GSH in Lycium barbarum L fruits was significantly affected by extracellular chemical environment during sample treatment. Extraction with DTNB as the simultaneous derivatizing agent inhibited the transformation or decomposition of GSH due to immediate derivatization during sample treatment. Quantitative determination results indicated that Lycium barbarum L fruits contained GSH in the range of (6.56 ±0.54) mg/g, which was one of the most GSH-abundant plant species. Key words ：glutathione ；5,5 ＇-dithio-bis-nitrobenzoic acid (DTNB)；Lycium barbarum L ；mass spectroscopy ； simultaneous derivatization 中图分类号：O657 文献标识码：A 文章编号：1002-6630(2011)06-0250-06 谷胱甘肽(glutathione ，G