dfmg调控氧化损伤vecs tlr4表达对vsmcs增殖与迁的影响.docxVIP

优秀毕业论文 精品参考文献资料 ABSTRACTobjective ABSTRACT objective： To observe whether the change of TLR4 expression in the oxidation·damaged vascular endothelial cells(VECs)regulated by 7-Difluoromethyl-5，4’一dimeth- oxygenistein(DFMG)call influence the proliferation and migration of vascular smooth muscle cells(VSMCs)． Method： QThe VECs．VSMCs coculture model was established，and LPC Was used for inducing the injury of VECs．CCK一8 assay and Transwell—migration metllod was used to determine the proliferation and migration of VSMCs． ELISA was used to analyze the amount of interleukin 6(IL一6)and human tumor necrosis factor-a(TNF．a)in the VECs supernatant，and Western blot and Quantitative real．time PCR was used to detect TLR4 protein and gene expression of VECs in the injured VECs--VSMCs coculture model· ②VECs were pre—incubated with DFMG at different concentrations and different times，and CCK．8 assay Was used again to determine the proliferation of VSMCs in the injured VECs-VSMCs coculture model·Then， VECs is processed by DFMG，TLR4 overexpression or silencing，the proliferation and migration of VSMCs in the injured VECs-VSMCs coculture model was analysed by CCK一8 assay and Transwell-migration method． Resuits： 万方数据 万方数据 万方数据 ①LPC—induced ①LPC—induced injured VECs caused the proliferation and migration of VSMCs． @The amount of LL-6 and TNF．a in the VECs supernatant increased with the LPC concentration in the injured VECs—VSMCs coculture model，and the 300M group became the model ofthe following experiment． ⑨The injured VECsWSMCs coculture group had a higher TLR4 protein and gene expression in VECs than the normal VECs-VSMCs coculture group． @The proliferation of VSMCs in the injured VECs-VSMCs coculture model was influenced by DFMG on VECs in a time--and concentration--dependent manner，and l rtM and 1 2h was selected as the best treatment concentration and time of DFMG． (亘)TLR4 over expression has a similar role with LPC，which Can promote the proliferation and migration of VSMCs in CO—c