基于性状—显微—DNA条形码联用巴西.PDFVIP

环球中医药2015年11月第8卷第11期摇 Global Traditional Chinese Medicine,November2015,Vol郾8,No郾11 摇1309 ·巴西草药研究专题 · 基于性状—显微—DNA条形码联用的巴西 牛至属植物药HORTELA 基原鉴定研究 余春霞摇 臧艺玫摇 顾选摇 吴浩忠摇 马长华摇 韩丽摇 刘春生摇 赵百孝 揖摘要铱摇 目的摇 利用性状—显微—DNA条形码联用的方法对巴西牛至属植物药HORTELA 进 行基原鉴定研究。 方法摇 应用性状鉴定的方法初步对药材进行真伪鉴别;再应用显微鉴定的方法 验证性状鉴定结果;最后提取样品DNA,扩增ITS序列,利用NCBI数据库中的BLAST功能分析相似 度,对样品品种进行DNA条形码鉴定。 结果摇 性状—显微—DNA条形码综合分析结果表明,该样 品为牛至属植物Origanum elongatum(Bonnet) Emb. Maire.的干燥地上部分。 结论摇 HORTELA 可 能为多基原植物类药材,其来源为牛至属 Origanum elongatum (Bonnet) Emb. Maire.和薄荷属 Mentha rotundifolia (Linn.) Huds.干燥地上部分。 揖关键词铱摇 牛至属;摇 基原鉴定;摇 DNA条形码 揖中图分类号铱摇 R284.1摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2015郾11郾009 The variety identification ofHORTELA in Brazil based on a combined analysis of macroscopic鄄mi鄄 croscopic鄄DNA barcoding摇 YU Chun鄄xia,ZANG Yi鄄mei, GU Xuan,et al. Henan Provincefor Drug Evaluation Certification Center,Zhengzhou450004,China Corresponding author:LIUChun鄄sheng,E鄄mail:max_liucs@263.net 揖Abstract铱摇 Objective摇 To study the variety identification of HORTELA of Origanum Linn. in Brazil with acombinedanalysisof macroscopic鄄microscopic鄄DNAbarcoding. Methods摇 Macroscopiciden鄄 tification was used to identify the authenticity of samplespreliminarily;thentheresultswasverifiedby mi鄄 croscopic identification;finally,ITS sequences were amplified by PCR and sequenced, and BLAST in NCBI (basic local alignment search tool) database was used to calculate the similarity of sequences to identify variety of samples. Results摇 The combined analysis of macroscopic鄄microscopic鄄DNA barcoding indicated that Hortela collectedfrom market in Brazil is the dried overground part of Origanum elongatum (Bonnet) Emb. Maire. Conclusion摇 HORTELA may be amulti鄄sourceherb ,which originatesfromthe driedovergroundpart of Origanumelongatum(Bonnet)Emb. MaireandMentharotundifolia (Linn.)Huds. 揖Key words铱摇 Origanum Linn. ;摇 Variety identification