嗜酸氧化亚铁硫时杆菌atcc23270碱激反应的全基因组表达谱研究-微生物学专业毕业论文.docxVIP

A B A B S T R A C T A s o n e o f th e a c id o P h ilic b io le a c h in g b a e te ria l sP e e ie s ， A cidl’thio bacillus fe rro ox l’da ns (A . fe rro ox l’da ns) 15 P ron e to gro w in e n v iro n m e n t o f P H a t a b o u t 2 .0 . In P ra c tie a l in d u stry， it 15 P ro v e d th a t th e h ig h ly a e id ie e n v iro n m e n t 15 h a rd ly m a in ta in a b le d u e to th e d is so lu tio n o f a lk a lin e m atrix an d fu rth e r to im P e d e th e b io le a e h in g e ffi e ie n e y o f A . fe rro ox ida ns. T h erefore，it 15 very v alu ab le to exP lore how to d om estieate th e b io le a e h in g b a e te ria o f th is k in d ，a n d it 15 th e o re tie a lly m e a n in g fu l to in v e s tig a te h o w th e y e x P re ss a n d re g u late a t w h o le 一g e n o m e le v e l in re sP o n se to P H u P sh o c k . In th is stu d y，w e c o n stru c te d th e w h o le 一g e n o m e o lig o n u e le o tid e a rra y b ased o n th e w ho le 3 ，2 17 O RF s o f A .fe rro ox ida ns AT C C 232 70 g eno m e. T h e tra n sc riP to m e a n a ly sis o f th is b a e te ria l sP e e ie s in re sP o n s e to P H u P sh o e k w a s c o n d u c te d b y th e w h o le 一g e n o m e a rra y， a n d illu m in a te its e x P re s s io n d yn a m ie s ， fu rth e r to e x P lo re th e re sP o n se m e c h a n is m to su rm u n d in g stre ss . In th is stu d y，a w h o le 一g e n o m e 5 0 一m e r-b a se d o lig o n u c le o tid e m ie ro a rra y w a s e ffe e tiv e ly d e v e lo P e d e o v e rin g 一9 7 % o f th e to ta l P re d ic te d P rotein 一eneod ing oP en readin g fram es (O RF s) in A . fe rro ox ida ns AT C C 2 3 2 7 0 . B a se d o n art ific ia l o lig o nu e le o tid e P ro b e s ，o u r re su lts sh o w e d th a t th e o P tim a l h y b rid iz atio n te m P e ra tu re w a s a t 4 5 oC . S P e e ifie ity te sts w it