一种微孔板生物毒性快速检测技术的建立-环境工程专业毕洗业论文.docxVIP

ABSTRACTThis ABSTRACT This paper described a multi—species 1 8一h microbial assay,TOXPlate，which developed here for acute toxicity assessment of pure chemicals and environmental samples．A tetrazolium dye，2,3，5一triphenyltetrazolium chloride(TTC)，WaS used as an indicator to test bacterial growth inhibition in each well of microplate．Measurement of the growth of the organisms exposed to a range of concentrations of tested chemicals is determined by the reduction of TTC After being tested by several chemicals，eleven different indigenous strains WaS selected over dozens of candidates which were all