桑叶多酚提取工艺优化研究沈维治.pdfVIP

桑叶多酚提取工艺的优化研究 1，2 *1，2 1 2 1，2 沈维治 ，廖森泰 ，刘吉平 ，邹宇晓 ，李妍 1 2 （华南农业大学动物科学院，广州 510642； 广东省农业科学院蚕业 与农产品加工研究所，广东省农产品加工重点实验室，广州 510610） 摘 要 文运用二次回归正交旋转组合设计法研究了乙醇浓度、料液比、pH 值对桑叶 酚得率的影 响，建立了具有良好预测性能的提取条件数学模型，确定了最优提取条件。试验结果表明，在 试验 范围内对桑叶 酚得率影响作用大小的顺序为：pH 值料液比＞乙醇浓度。最优提取工艺条件为：溶剂 为70%的乙醇，料液比为1：40，pH 为4， 条件下提取桑叶 酚含量为16.11mg/g。 关键词 桑叶； 酚；二次回归正交旋转组合设计；提取工艺；优化 中图分类号 文献标识码 A 文章编号 The Optimization study on extraction processe of Polyphenol from Mulberry leaves 1,2 1,2 1 2 1,2 SHEN Wei-Zhi LIAO Sen-Tai , LIU Ji-Ping , ZOU Yu-Xiao , LI Yan 1 （College of Animal Science,South China Agricultural University,Guangzhou 510642,China 2Institute of Sericulture and Agro-food Science and Technology,Guangdong Academy of Agriculture Sciences, Key Laboratory of Agro-food Science and Technology of Guangdong,Guangzhou 510610,China ） Abstract By means of orthogonally rotational combination design, the effect of ethanol concentration, solid-liquid ratio, pH on the yield of mulberry leave polyphenol were researched . A quadric regression model of polyphenol yield was established, the model was significantly fit well and the optimum combination was obtained. The results indicated that the yield of mulberry leave polyphenol was affected obviously in decreasing order by pH , solid-liquid ratio, and ethanol concentration .The optimum condition for the extraction is as following: extracting solvent 70 percent ethanol, solid-liquid ratio 1:40, pH 4, the yield