细胞过继免疫治疗肿瘤临床研究进展.pptVIP

* * * * * Whole blood (70 ml) was collected in BD Vacutainer blood collection tubes with sodium heparin and directly centrifuged to isolate PBMCs. PBMCs were stimulated with 5-mM zoledronic acid (Novartis, Basel, Switzerland) in AlyS203-gd medium (Cell Science and Technology Institute, Sendai, Japan) containing 1000 IU ml1 human recombinant IL-2 and 10% autologous serum at the beginning of the culture. * * Schemes for adoptive transfer of autologous, vaccine-primed, in vitro–expanded T cells. Patients are primed with tumor vaccine followed by lymphocyte harvest. Autologous T cells are harvest