基于硅溶胶-凝胶的核黄素分子印迹传感器的研究.docVIP

PAGE 基于硅溶胶-凝胶的核黄素分子印迹传感器的研究 摘要 以玻碳电极为基质材料，以四甲氧基硅烷、苯基三甲氧基硅烷为单体，以核黄素为模板分子，通过循环伏安法制得核黄素分子印迹传感器。利用差分脉冲伏安技术对分子印迹传感器的印迹效果和选择性等因素进行了研究。结果表明，硅溶胶-凝胶分子印迹膜具有较高的稳定性，核黄素分子与印迹膜相互作用的可逆性好；该分子印迹传感器对核黄素有显著的印迹效应和较高的选择性，核黄素浓度在0.01～1.0 QUOTE mol.L-1范围内与峰电流成正比。 关键词：核黄素，电化学传感器，分子印迹术,硅溶胶-凝胶分子印迹膜 The research based on silica sol - gel riboflavin molecularly imprinted sensor Abstract With glassy carbon electrode as matrix material, TMOS, PTMOS as monomer, using riboflavin as template molecule, and riboflavin molecular imprinting sensor is obtained by cyclic voltammetry method. Using the differential pulse voltammetric techniques, the imprinting effect and selectivity of molecularly imprinted sensor were studied. The results showed that the silica sol - gel molecular imprinted membrane has a high stability, the reversibility of the riboflavin molecule interaction with the imprinted membrane is good; the molecularly imprinted sensor for riboflavin has obvious imprinting effect and higher selectivity, riboflavin concentration range 1.0×10-6 QUOTE mol.L-1 from 1.0×10-8 mol.L-1 is proportional to the peak current. Key word: Riboflavin, electrochemical sensors, molecular imprinting technique, silica sol - gel molecularly imprinted membrane 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc326249752 1 引言 PAGEREF _Toc326249752 \h 1 HYPERLINK \l _Toc326249753 2实验部分 PAGEREF _Toc326249753 \h 3 HYPERLINK \l _Toc326249754 2.1 仪器 PAGEREF _Toc326249754 \h 3 HYPERLINK \l _Toc326249756 2.2 试剂 PAGEREF _Toc326249756 \h 3 HYPERLINK \l _Toc326249757 2.3 硅溶胶-凝胶分子印迹膜修饰电极的制备 PAGEREF _Toc326249757 \h 3 HYPERLINK \l _Toc326249758 2.3.1 玻碳电极的预处理 PAGEREF _Toc326249758 \h 3 HYPERLINK \l _Toc326249759 2.3.2 印迹溶胶的制备 PAGEREF _Toc326249759 \h 3 HYPERLINK \l _Toc326249760 2.3.3 硅溶胶-凝胶分子印迹膜修饰电极的制备 PAGEREF _Toc326249760 \h 3 HYPERLINK \l _Toc326249761 2.3.4 核黄素分子印迹膜电极印迹效应测定 PAGEREF _Toc326249761 \h 4 HYPERLINK \l _Toc326249762 3结果与讨论 PAGEREF _Toc326249762 \h 4 HYPERLINK \l _Toc326249763 3.1 硅溶胶-凝胶分子印迹膜修饰