2酶学与酶工程第二去章学生.pptVIP

* 速度方程式： V = Vmax [S] Km (1+ [ I ] [ Ki ] ) +[S] Km′ (表观米式常数) Ki: 抑制剂常数 * Vmax v [S] （不变） Km Km? (变大) With competitive inhibitor No inhibitor ? 竞争性抑制曲线 Fig. Competitive inhibition * Vmax Km V 1 = ) [ I ] [ Ki ] (1+ + Vmax 1 [S] 1 1/[S] [ I ] 1/v [ I ] [ Ki ] -Km ( 1+ ) -1 -1/Km No inhibitor With competitive inhibitor Fig. Lineweaver-Burk plot of competitive inhibition. 2. 非竞争性抑制Noncompetitive * * V = Km + [S] Vmax [S] [ I ] [ Ki ] 1+ Vmax? * [S] v Vmax? Vmax （变小） Km (不变) No inhibitor With noncompetitive inhibitor * [S] 1 Vmax Km V 1 = ) [ I ] [ Ki ] (1+ + Vmax 1 ) [ I ] [ Ki ] (1+ No