vitc，e对60coγ线照射家兔血清和肝脏sod和mda影响.docVIP

Vit.C,E对60 Coy线照射家兔血清和肝脏影响 姓 名： 2014年6月25日 Vit.C, E对60 Co丫线照射家兔血清和肝脏SOD和MDA影响 ：梁欣，海春旭，秦绪军，张晓迪， 赵康涛，刘瑞，陈宏莉，沈学锋 关键词 辐射损伤 关键词：维生素C;维生素E;辐射损伤;抗氧化;超氧化物歧化酶;丙二醛 :目的探讨Vit.C, E对60 CoY线照射家兔的保护作用以及剂量效 应关系.方法中国本兔随机分为12组，每组5只.Vit.C5组iv,剂量分别为10, 20,40,80 和 160mgkg-l , Vit.E 汕剂 ig5 组，剂量分别为 5,10,20,40 和 80mgkg-l . 第3日进行60 CoY线（4.5Gy）照射家兔.第5日处死、剖腹取肝脏和腹腔静脉血, 分别测定超氧化物歧化酶（SOD）的催化活性和丙二醛（MDA）的含量变化.结 果辐射动物补充Vit.C和Vit.E组的肝SOD的催化活性与阴性对照组相比明显 升高（P0.01）, Vit.E的作用更明显，呈现了一定剂量效应关系;各Vit.C组肝脏 MDA的含量变化不显著（P0.01）,但血清MDA的含量轻微下降;各Vit.E组肝脏 和血清中的MDA的含量均显著降低（P0.01）,且呈现出一定的剂量效应关系.结 论单独使用Vit.C的抗氧化作用，有时还会加重损伤;单独使用Vit.E具有一定 的SOD活性保护和MDA清除作用，存在剂量效应关系，但对外周血细胞和免 疫细胞则无明显的保护作用. Keywords:vitamin C;vitamin E;radiation injury;antioxida-tion;SOD;MDA Abstract:AIM To study the dose-effects relationship be-tween Vit.C，Vit.E, and lipid peroxidation through the mod-el of rabbit injured by radiation.METHODS Chinese rab-bits were chosen as the experimental animals and were ran-domly divided into twelve groups which each contained five.They were negative control group，positive group, and ten trial groups of Vit.C，Vit.E.The trial groups contained five different dosage of Vit.C and five different dosage of Vit.E.Peanut oil was selected as diluent which had been getting rid of Vit.E.The five groups were supplied by injection of vein with the dosages of Vit.ClO, 20，40，80andl60mgkg-l every day respectively.The five Vit.E groups were supplied by gavages with the dosage of Vit.E5，10, 20, 40and80mgkg-l，every day espectively too.On the third day all groups, except negative control group, were radiated by60 Coyray with the dose of4.5Gy.Then they were fed as before，and on the sixth day all rabbits were sacrificed.Their blood and livers were removed for the analysis of changes of catalytic activity of SOD and content of MDA.RESULTS After the radiation, the catalytic activities of hepatic SOD in the radiation groups were higher than those in the negative control group (P0.01 )，and the trial groups of Vit.C and Vit.E showed some prot