草地早熟禾对立枯丝核菌侵染的生理响应.PDFVIP

中国农学通报 2015,31(10): 198-203 Chinese Agricultural Science Bulletin 草地早熟禾对立枯丝核菌侵染的生理响应 欧阳路平，尹淑霞 （北京林业大学草坪研究所，北京100083） 摘 要：为探索立枯丝核菌(Rhizoctonia solani)侵染后草地早熟禾(Poa pratensis L.)的生理响应，以2 个对 褐斑病抗性不同的草地早熟禾品种‘午夜’和‘蓝孔雀’为材料，在接种立枯丝核菌后，统计了草坪草的发 病率和病情指数，并测定分析不同时间点草坪草叶片内的相对电导率值、丙二醛含量、脯氨酸含量、叶绿 素含量等生理指标的变化规律。结果表明：与接种前相比，接种后的草叶随着时间的推移，其病情指数、 相对电导率、丙二醛和脯氨酸含量都显著增大，而叶绿素含量显著降低。其中，易感病品种‘蓝孔雀’的 病情指数、相对电导率、丙二醛和脯氨酸含量的增幅均高于抗病品种‘午夜’，而且前者叶绿素含量的降 幅也大于后者。相关分析表明，丙二醛含量、脯氨酸含量和相对电导率与病情指数的变化呈显著正相 关，叶绿素含量与病情指数呈显著负相关。 关键词：草地早熟禾；立枯丝核菌侵染；生理响应 中图分类号：S182 文献标志码：A 论文编号：2014-2639 PhysiologicalResponseofKentuckyBluegrasstotheInoculationofRhizoctoniasolani OuyangLuping,YinShuxia (InstituteofTurfgrassScience,BeijingForestryUniversity,Beijing100083) Abstract:Toexplorethephysiologicalresponseofkentuckybluegrass(Poapratensis L.)totheinoculationof Rhizoctoniasolani,twokentuckybluegrasscultivars, ‘Midnight’and ‘BluePeacock’,whichhaveexhibited resistantandsusceptibletoRhizoctoniasolaniwereinoculatedby Rhizoctoniasolani.Duringtheinoculation period,thediseaseincidenceanddiseaseindexwerecalculatedandtherelativeconductivity,contentsof MDA,prolineandchlorophyllintheleaftissuesofturfgrassesweretestedandanalyzedcomparatively.The resultsindicatedthatsomeparametersincludingdiseaseindex,relativeconductivity,contentsofMDA,proline and chlorophyll increased after inoculation while chlorophyll content gradually decreased compared to no-inoculation.Duringthe7daysafterinoculation,diseaseindex,relativeconductivity,contentsofMDAand prolineincreasedhigherin ‘BluePeacock’thanthatin ‘Midnight’.Moreover,thedegreeofreductionof chlorophyllcontentwashigherin ‘BluePeacock’thanthatin ‘Midnight’.Correlationanalysisshowedthat significant positiv