六种冬青属苦丁茶叶片中绿原酸含量的RP―HPLC分析.docVIP

六种冬青属苦丁茶叶片中绿原酸含量的RP-HPLC 分析 采用超声提取和RP-HPLC法分析了 6种冬青属苦丁 茶新干燥的功能叶及苦丁茶冬青植物在不同发育阶段、不同 地域、野生与栽培材料、不同存放时间下功能叶中的绿原酸 含量。结果表明，不同样品材料中的绿原酸含量由大到小排 序依次为苦丁茶冬青、霍山冬青、大叶冬青、五棱苦丁茶、 华中枸骨、枸骨；新干燥的成熟叶样品在室温下存放2个月 后绿原酸的含量显著下降；在野生的苦丁茶冬青叶片不同发 育阶段中，一般是以老叶样品中的绿原酸含量高；野生苦丁 茶冬青植株叶片中绿原酸的含量要高于栽培植株叶片中绿 原酸的含量。6种冬青属苦丁茶叶片样品中的绿原酸含量存 在较大的种间差异。 关键词：冬青属苦丁茶；绿原酸；反相高效液相色谱；资 源利用 TS275.2 A 0439-8114 (2014) 12-2889-03 Comparison of Chlorogenic Acid in Leaves from Six Ilex Species Used as Kudingcha by RP-HPLC TIAN Jian-pingla, lb, 2，LI Juan-linglc , HU Yuan-yanld, LIU Guo-minla, lb (1. a. Kudingcha Research Institute, b. College of Agriculture, c. Horticulture and Landscape College, d. Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228，China；2. Department of Pharmacy, Hainan Medical College, Haikou 571101， China) Abstract： Chlorogenic acid in the mature leaf was extracted ultrasonically and analyzed by RP-HPLC from six Ilex species and I.kudingcha in different developing stage, region and storaging time, wild and cultivated samples. The results showed that the content of chlorogenic acid in fresh mature leaves of six species was I. kudingcha〉 I. huoshanesis I. latifolia I. centrochinensis I. pentagona I. cornuta. The significant decrease of chlorogenic acid in dried fresh leaf was observed after two months. In general，its content in old leaf was highest among different developing stage, and the content of wild leaf was higher than cultivation one. There were large differences among six Ilex species in the contents of chlorogenic acid. Key words : Kudingcha species in Ilex L ; chlorogenic acid； RP-HPLC； resource utilization 华中枸骨（I.苦丁茶是我国民间广泛用于制备代茶饮品（或茶药两 用）的一大类代茶植物的统称。其原植物来源广泛，涉及12 科13属，至少有36种之多（含变种和变型）［H3］。冬青 属苦丁茶是我国两大类最重要的苦丁茶之一。该类苦丁茶主 要包括苦丁茶冬青（Ilex kudingcha C. J. Tseng）,大叶冬 青（I. latifolia Thunb）,枸骨（I. cornuta Lindl），霍 山冬青（I. huoshanesis Y. H. He,五棱苦丁茶 华中枸骨（I. S. K. chen Y. X. Feng et C. F. Liang centrochinensis S. Y. Hu）等6个物种。这些苦丁茶物种主 要分布于我国广东、广西、海南、浙江、安徽和贵州等省区 ［4-6］。根据种植