‘夏日阳光’番茄侧芽离体培养快繁技术.PDFVIP

中国农学通报 2016,32(34):49-54 Chinese Agricultural Science Bulletin ‘夏日阳光’番茄侧芽离体培养快繁技术 赖呈纯，潘 红，范丽华，谢鸿根，黄贤贵 （福建省农业科学院农业工程技术研究所，福州350003） 摘 要：‘夏日阳光’番茄是近年来引进的高端品种，丰产性好、品质高，深受市场的青睐，但其种子为F1 代杂种，价格昂贵，很难在市场上大面积推广。利用植物离体培养技术快速繁殖‘夏日阳光’番茄种苗， 是生产上推广该品种的一条可行途径。为快速建立‘夏日阳光’番茄无菌株系以及获得其试管芽苗的高 效增殖，以‘夏日阳光’番茄侧芽为材料，探讨其离体培养过程中的关键技术。试验结果表明，采用0.1% HgCl 溶液(w/v)加2 滴土温20 消毒8 min 的处理方式消毒后，培养在MS+ 0.1 mg/L BA+ 0.1 mg/L IBA 培 2 养基中，可以较快速地建立‘夏日阳光’番茄无菌株系，成苗率为46.7% ；继代增殖适宜的培养基配方为 MS+ 0.5 mg/L BA+ 0.1 mg/L IBA ，增殖系数可达4.3 ，并且芽苗生长健壮，为其种苗的规模化繁育提供技 术基础。 关键词：番茄；侧芽；外植体；增殖；快繁技术 中图分类号：S641.2 ，Q813.1 文献标志码：A 论文编号：cas InvitroCultureTechniqueforRapidPropagationofLateralBudof Lycopersiconesculentum ‘Xiariyangguang’ LaiChengchun,PanHong,FanLihua,XieHonggen,HuangXiangui (InstituteofAgriculturalEngineeringandTechnology,FujianAcademyofAgriculturalSciences,Fuzhou350003) Abstract: Lycopersicon esculentum ‘Xiariyangguang’isanimportedhigh-leveltomatovarietyrecently, whichgetsthefavorofmarketbecauseofhighyieldandhighquality.However,itisdifficulttobeplantedin largescaleformarketing becauseofitsexpensiveseeds,whichareallhybridF generation.Baseontheplant1 in vitro culturetechniques,theseedlingsof Lycopersicon esculentum ‘Xiariyangguang’willbepropagated rapidly.Therefore,itisafeasiblewayfor promoting thisvarietyinproduction.Inordertorapidly establish asepticlinesof Lycopersicon esculentum ‘Xiariyangguang’andobtainhigh-performanceproliferationofits shoots in vitro,thekeytechniqueswereinvestigatedbyusingitslateralbudsasmaterialsduringits in vitro culture.Theresultsshowedthatafteritsexplantwasdisinfectedbyusing 0.1%mercuricchloridewithsome Tween 20 treatmentfor 8 min,theasepticlinesof Lycopersicon esculentum ‘Xiari