丁苯酞动员内皮祖细胞治疗缺血性卒中大鼠的实验分析.pdfVIP

丁苯酞动员内皮祖细胞治疗缺血性卒中大鼠的实验研究 英文摘 ExperimentalstudyonButylphthalidetreatingischemic strokebymobilizationofendothelialprogenitorcells Abstract Objective: To explore the efficacy and mechanism of Butylphthalide on ischemic stroke, effect of different doses of Butylphthalide (NBP) on the neurological function, circulating endothelial progenitor cells (EPCs) and neurogenesis in the ischemia region wereobservedinmiddlecerebralarteryocclusion(MCAO)/reperfusionrat. Method: 48 male Sprague-Dawley rats weighted (250±20)g were selected in this study,whichwererandomlydividedintofourgroups,ie.shamgroup,modelcontrolgroup, low dose NBP group and high dose NBP group, and 12 animals in each group. Neck vessels were isolatedbut thread embolismwas not inserted in sham group. On the other three groups, thread embolism method was used to prepare middle cerebral artery occlusion (MCAO)/ reperfusion animal model. Two rats in both sham group and model controlgroupwereperformedTTCstainingtoobservemorphologicalchangesinischemic area. The rest rats were respectively gavaged with two doses ofNBP or control placebo (soybeanoil)(2ml/d)for5days.Neurologicalfunctionofallratswereassessedeveryday. Peripheral blood was collected before and after treatment to investigate the level of circulating EPCs with flow cytometry. Immunochemical staining of CD31 and vWF (markersofvascularendothelialcells)wasperformedtoobserveangiogenesisafter5days oftreatment.AllthedatawereanalyzedbyGraphPad5.0softwareanalysissystem. Results： 1 Hemisphere onthe sideofcarotidarteryinsertedwiththread embolismdisplayed ） palecolorandhemisphereontheothersidedisplayedredcolor. 2 On day 1after treatment, neurological function score reduced in low doseNBP ） group (P0.05), and it significantlyreduced in high dose group (P0.01).The scorewas significantlydecreasedonday3aftertreatme