单侧横突拉簧加压建立兔脊柱侧凸模型.PDFVIP

·594 · doi 10.3969/j.issn. 1673-4254.2015.04.27 J South Med Univ, 2015, 35(4): 594-597 基础研究 单侧横突拉簧加压建立兔脊柱侧凸单侧横突拉簧加压建立兔脊柱侧凸模型模型 李伦超，刘 湘，朱青安，黄志平，任海龙，郑 帅，李广军，杨德鸿，王吉兴 南方医科大学南方医院脊柱骨科，广东 广州 510515 摘要：目的利用单侧横突拉簧加压法建立兔脊柱侧凸模型。方法 两月龄新西兰白兔10只，手术予L 、L 右侧横突挂载拉簧。 2 5 术前、术后1、4 及8 周摄X 线正侧位片，测量Cobb 角及拉簧长度，术后8 周摄片后，随机将10只新西兰白兔分为两组：取拉簧 （Spring removal, SR）组和保留拉簧组（Spring keeping, SK）各5 只，取出SR组兔子体内拉簧，于9 周和12周摄X 线正侧位片，测 量Cobb 角及后凸角。12周后取所有兔顶椎及其上线椎体行Micro-CT 扫描，记录顶椎上下终板的骨体积分数（BV/TV）。用方 差分析或非参数检验及Student t 检验对实验结果进行统计学分析。结果 至实验结束时，兔全部存活，Cobb 角均大于10°。术 后1、4、8周不同时间点Cobb 角及后凸角都大于术前（P0.05）。术后8周，SR组与SK组的Cobb 角、后凸角以及拉簧长度均无明 显差异（P0.05），取出拉簧后至12周，SK组的Cobb 角及后凸角都较SR组要大（P0.05），期间SR组的Cobb 角及后凸角都逐渐 减小。Micro-CT 结果示凹侧终板骨体积分数要大于凸侧。整个实验过程中，拉簧形变量（ΔL）均无统计学差异（P0.05）。结论 单侧横突拉簧加压模型是一种可重复率高、简单易操作且实验周期短的动物脊柱侧凸模型。 关键词：脊柱侧凸；模型/新西兰兔；拉簧；不对称载荷 Establishment of a rabbit model of scoliosis induced by asymmetric load using springs LI Lunchao, LIU Xiang, ZHU Qingan, HUANG Zhiping, REN Hailong, ZHENG Shuai, LI Guangjun, YANG Dehong, WANG Jixing Department of Spine Surgery, Nanfang Hospital, Southern Medical University ，Guangzhou 510515 ，China Abstract: Objective To establish rabbit model of scoliosis induced with stable asymmetric lumbar loads. Methods Scoliosis was induced in 10 two-month-old New Zealand rabbits using 316L stainless steel springs placed between the unilateral transverse processes of L2 and L5. Serial radiographs were documented before and at 1, 4, 8, 9 and 12 weeks after the operation. At weeks, the rabbits were randomly divided into SR group (n=5) with the spring removed and SK group (n=5) without spring removal. Results All the rabbits survived the experiment with Cobb angle all greater than 10°at the end of the experiment. Significant changes were found in the Cobb angles and kyphotic