多重标的悬浮阵列系统.PDFVIP

多重標的偵測技術之創新與突破 Bio-Plex Multiplex Suspension Array System 蔡文評(WenPing Tsai) 產品應用專員 Bio-Rad Laboratories Taiwan Ltd. 103年4月14 日星期一 Contents Overview • Multi-targets Detection (Multiplex) • Bio-Plex suspension array Technology • Bio-Plex suspension array system 1 Contents Overview • Multi-targets Detection (Multiplex) • Bio-Plex suspension array Technology • Bio-Plex suspension array system 多重偵測技術平臺 Real Time PCR Microarray BioPlex Suspension Array 2 Trend Towards Multiplexing Benefits of Multiplexing • Increase amount of information from one experiment • Decrease volume requirements (mouse samples, tumor biopsies, newborn screening) • Reveals complex relationships and pathways of biomolecules • Reduce reagent, expense and labor Multiplexing Increases Throughput 3 主要應用領域 • 基因表現(Protein Level), 可定量 –藥物作用 – 腫瘤指標 – 基因調控 – 基因治療 – GMO • 病原菌偵測(Nucleic Acid), high sensitivity – 多重病原菌同時偵測 – 適合大量檢體篩選 – 定性, 定量同時完成 –藥物治療成效的監控 • SNP 分型(Nucleic Acid) Micro Array • Micro array: High entrance barrier – One chip, four sample – Overnight incubation – Reproducibility