柔嫩艾美耳球虫河北株EtMIC-2基因真核表达载体的构建.pdfVIP

ABSTRACT Thechickencoccidiosisisa diseasecausedoneormore protozoanparasitic by virulentEimeria incecumwhichleadtoserious tothe speciesparasitize damage E．tenellamicroneme-2 secretedfromthe industry．The protein(EtMic2)is poultry microneme itinvolvedinthe ofthe alsoitCan organelles，and processinvasion，and causethechicken reaction．Interleu oneofimmune bodyimmunity kin-2(IL-2)is whichCanenhanceimmune andhostimmune regulationcytokines protection DNAvaccineofHebeistrain this constructedrecombinant response．Instudy,we and recombinantwereidentifiedrestriction EtMIC2 IL-2，the plasmids by enzyme andtransfectedinto293T wereverified cells，the by digestion targetgenes expression wasdividedintothe sections： RT-PCRmethod．The following study andbioinformaticsofIL-2 inchicken 1．Cloning analysisgene The oftheresearchwe a of to part designedpairspecificprimersaccording intheIL-2mRNA usedtheRT-PCPmethodfor GenBank sequences，then cloning theIL一2 fromchicken．The showedthatthefull of gene sequenceanalysis length was432 a432 132 readingframe，andencoding IL一29ene