分子生物学实验技术总结.pptVIP

Techniques Summary Li Xiangyun 2011-3-28 * Select appropriate vector Get the target DNA sequence PCR primer design Obtain cell or tissue PCR amplification Restriction, ligation and transformation Screen positive clone Sequencing Vector construction procedure Select appropriate vector Human cytomegalovirus (CMV) immediate-early promoter/enhancer: Permits efficient, high-level expression of your recombinant protein T7 promoter/priming site : Allows for in vitro transcription in the sense orientation and sequencing through the insert Multiple cloning site in forward or r