GO分析的简介与示例.PDFVIP

GO分析的简介与示例 a Brief Introduction and Application of Gene Ontology G14 自振滔张书孔祥悦黄圣楠 2016.01.16 The Gene Ontology (GO) For consistent descriptions of gene products across databases The Gene Ontology (GO) GO terms GO terms -simple hierarchical controlled vacabulary -three organizing GO principles: Molecular Function Biological Process Cellular Component Molecular Function -describes activities that occur at the molecular level -represent activities rather than the entities (molecules or complexes) that perform the actions e.g. catalytic activity adenylate cyclase activity Biological Process -describes a series of events accomplished by one or more organized assemblies of molecular functions e.g. signal transduction alpha-glucoside transport -biological process VS molecular function biological process must have more than one distinct steps. Cellular Component -a component of a cell that is part of a larger object e.g. rough endoplasmic reticulum nucleus What does the gene product do ? Molecular Function Where does it act? Cellular Component Why does it perform these activities? Biological Process Ontology Structure -Is_a GO:0015758 : glucose transport is a GO:0015749 : monosaccharide transport -Part_of GO:0031966 : mitochondrial membrane part of GO:0005740 : mitochondrial envelope -Regulates GO:0006916 : anti-apoptosis regulates GO:0012501 : programmed cell death GO annotation -annotation is the process of assigning GO terms to gene products -source literature another database computational analysis GO Annotation File Format GO Annotation File Example GO Tools and Data Analysis GO BiNGO Background: Sleep Universal: C.elegant…human Higher cognitive function: memory, dream, cognition Drosophila sleep: 5 mins stationary – Sleep length screening: target