《克东腐乳发酵生产中优势菌株细胞融合方法初探》》-毕业论文.docVIP

PAGE 精品 精品 齐 齐 哈 尔 大 学 毕 业 论 文 题 目： 克东腐乳发酵生产中优势菌株 细胞融合方法初探 学 生 姓 名： 专 业 班 级： 指 导 教 师： 摘 要 本文以克东腐乳中藤黄微球菌（micrococcusluteus）QSY和QSG为研究对象，以青霉素（Penicillin）、链霉素（Streptomycin）和红霉素（Erythromycin）、白霉素（Kitasamycin）、卡那霉素（Kanamycin）、氯霉素（Chloromycetin）、庆大霉素（Gentamicin）、螺旋霉素（Spiramycin）、土霉素（Terramycin）和利福平（Rifampicin）10种抗生素分别作为两株菌的抗性标记。又分别从溶菌酶浓度、酶解时间、酶解温度、酶解pH值对这两株菌原生质体形成和再生的影响进行了初步探索。 实验结果表明：40μg/mL的青霉素和140μg/mL的链霉素可作为两株菌的抗性标记。在相同条件下，QSG菌株的最适溶菌酶浓度为3mg/mL，酶解时间为60min，酶解温度为37℃，酶解pH值6.5；QSY菌株的最适溶菌酶浓度为2mg/mL；酶解时间为30min，酶解温度为34℃，酶解pH值为6.5. 关键词：藤黄微球菌 抗性标记 原生质体 溶菌酶 Abstract This article take the Kedong fermented bean curd in garcinia hanburrii micrococcus (micrococcusluteus)QSY and QSG as the object of study, by penicillin (Penicillin), chain mildew element (Streptomycin) and erythromycin (Erythromycin), albomycin (Kitasamycin), card that mildew element (Kanamycin), chloromycetin (Chloromycetin), celebrates magnamycin (Gentamicin), spiral mildew element (Spiramycin), terramycin (Terramycin) and rifampin (Rifampicin)10 plants the antibiotic to take two fungi separately the resistant marks.Also from the bacteriolysin density, the enzymolysis time, the enzymolysis temperature, the enzymolysis pH value formed separately to these two fungus protoplast with the regeneration influence has carried on the preliminary exploration. The experimental result indicated that,40μg/mL penicillin and 140μg/mL the chain mildew element may take two fungi the resistant marks.Under the same condition, the QSG strain most suitable bacteriolysin density is 3mg/mL, the enzymolysis time is 60min, the enzymolysis temperature is 37℃, enzymolysis pH value 6.5; The QSY strain most suitable bacteriolysin density is 2mg/mL; The enzymolysis time is 30min, the enzymolysis temperature is 34℃, the enzymolysis pH value is 6.5. Key word: Garcinia hanburrii micrococcus Resistant mark Protoplast Bacteriolysin PAGE 精品 目 录 摘 要……………………………………………………………………………………Ⅰ Abstract ………