天然药物材料白和胶制备生物支架材料实验研究.PDFVIP

·2230 · 中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 16 期 2013 年 8 月 天然药物材料白及胶制备生物支架材料的实验研究 1 2 3 3* 侯绪浩 ，刘学蔚 ，彭凤梅 ，刘逢芹 1. 山东中医药大学药学院，山东 济南 250355 2. 山东大学口腔医学院，山东 济南 250012 3. 山东省千佛山医院，山东 济南 250014 摘 要：目的 探索采用中药天然成分制备多孔支架材料用于骨缺损修复生物材料的可行性，考察用于骨骼组织修复的白及 胶支架材料的制备方法及其性能。方法 从中药材白及中提取白及胶并精制，然后进行醛基化反应，与其他赋形剂共混、交 联，冷冻干燥得到支架材料。结果 利用水提醇沉法及酶法精制纯化能得到较高纯度的白及胶；经氧化后发生 Schiff 碱缩合 反应，形成良好的网状结构，冷冻干燥制得多孔支架材料。结论 以白及胶为主要原料制备的生物支架材料，各项性能表征 符合多孔支架材料要求。 关键词：白及胶；醛基化；交联；冷冻干燥；生物材料 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2013)16 - 2230 - 04 DOI: 10.7501/j.issn.0253-2670.2013.16.007 Study on preparation of natural medicine gum-bletilla scaffolds 1 2 3 3 HOU Xu-hao , LIU Xue-wei , PENG Feng-mei , LIU Feng-qin 1. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China 2. School of Stomatology, Shandong University, Jinan 250012, China 3. Qianfoshan Hospital, Jinan 250014, China Abstract: Objective To explore the feasibility of natural constituents in Chinese materia medica used for the preparation of porous scaffold material as bone repair biomaterials and for the tissue regeneration and to study the preparation method and the properties of gum-bletilla scaffolds. Methods Gum-bletilla was extracted from Bletilla Rhizoma, then purified, hydroformylated, mixed with other excipient, cross-linked, and lyophilized to get scaffolds. Results High-purity standard gum-bletilla could be obtained by water extraction, alcohol-precipitation, and enzymatic purification. After oxidation, the gum-bletilla had Schiff base condensation to form reticular conformation, which was made into porous materials by freeze-drying. Conclusion The scaffolds could be made of gum bletilla, which conforms to the requirements. Key words: gu