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信息检世索第五次作业、
PAGE
PAGE 5
《文献信息检索》综合作业3
要求：
1．通过实习，巩固所学的检索策略及资源类型。
2．写出所用检索词、检索表达式及命中篇数并复制粘贴2篇在文挡中。
3．课堂作业评点和分析时，希望积极参与教学互动。
一、医学数据库检索
1、Pubmed库
（1）检索福建中医药大学吴锦忠教授发文情况，分别以题录（Summary）和文摘（Abstrats）格式下载保存。
答案：检索式：Wu, Jinzhong[Full Author Name]
检索结果：3
文摘方式：
1. J Nat Med. 2013 Jan;67(1):17-26. doi: 10.1007/s11418-012-0639-x. Epub 2012 Mar 1.
Investigation of the extracts from Bidens pilosa Linn. var. radiata Sch. Bip. for
antioxidant activities and cytotoxicity against human tumor cells.
Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J.
Fujian University of Traditional Chinese Medicine, Fuzhou, Peoples Republic of
China.
The aim of this study was to evaluate antioxidant activities and cytotoxicity
against human tumor cell lines of extracts from Bidens pilosa Linn. var. radiata
Sch. Bip. (BP). Total phenolic and flavonoid contents of the extracts were
determined by ultraviolet spectrophotometry. Furthermore, the antioxidant
properties of different polarity fractions extracted from BP were evaluated using
DPPH and ABTS radical scavenging test and FRAP assay. The ethyl acetate fraction
(EE-BP) showed the highest antioxidant activity compared to other fractions. In
addition, the anti-proliferative activities of the extracts on four human tumor
cells, namely MCF-7, HepG2, MGC 803 and RKO, were investigated by MTT method. The
EE-BP displayed the most remarkable anti-proliferative effect against the tumor
cells, particularly RKO cell in dose- and time-dependent manner. The antioxidant
activities and cytotoxicity correlated highly with the total phenolic and
flavonoid contents, respectively. Furthermore, The active ingredient BP-6, namely
5,7,4-trihydroxy-3,3-dimethyl-flavonol, was isolated and purified with the
purity above 99.00% and content of 0.15% in EE-BP detected by HPLC, which could
significantly inhibit the proliferation of RKO cells with the IC(50) value of
6.66?μmol/l. In order to characterize the apoptotic RKO cells, flow cytometry and
DNA fragmentation assay were performed. Apoptotic
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