温度、光照及继代周期等对金叶复叶槭组培苗生长的影响.PDFVIP

中国农学通报 2012,28(25):209-212 Chinese Agricultural Science Bulletin 温度、光照及继代周期等对金叶复叶槭 组培苗生长的影响 孟月娥，李艳敏，赵秀山，王利民，王慧娟，张和臣，董晓宇 （河南省农业科学院园艺研究所，郑州450002 ） 摘 要：为了完善金叶复叶槭组培快繁技术、实现工厂化规模化生产，以其组培苗为试验材料，通过设置 不同的温度、光照、继代周期等，研究培养条件对其生长的影响。结果表明，温度、继代周期、光照条件对 苗高影响较大，差异均达到显著水平；同时发现继代周期显著影响叶片数量、光照时间对根生长系数影 响差异达到极显著水平。筛选出在30℃、4200 lx 的光照强度下，16 h/d 光照时间、继代周期35 天为‘金 叶复叶槭最佳培养条件。 关键词：金叶复叶槭；组织培养；温度；继代周期；光照 中图分类号：S792.35 文献标志码：A 论文编号：2012-1162 EffectofTemperature,SubcultureCycleandLightontheGrowthofAcernegundo ‘Aureomarginatum’inVitro Meng Yue ’e, Li Yanmin, Zhao Xiushan, Wang Limin, Wang Huijuan, Zhang Hechen, Dong Xiaoyu (HorticultureInstituteofHenanAcademyofAgriculturalSciences, Zhengzhou 450002) Abstract: This paper aimed to study the tissue culture and rapid propagation technology of Acer negundo ‘Aureomarginatum ’and provided basis for the implementation of scale production. The effects of different temperature, subculture cycle, illumination and other culture conditions on the growth of the Acer negundo ‘Aureomarginatum ’in vitro were investigated. The results showed that the differences by temperature, subculture cycle, light on the plantlet height reached the significant level, it was significantly affected on leaf number by subculture cycle, and on root growth coefficient by illumination time. The optimal culture conditions for Acer negundo ‘Aureomarginatum ’in vitro were at 30℃, 4200 lx light intensity, 16 h/d illumination time, and the subculture cycle was 35 days. Keywords:Acer negundo ‘Aureomarginatum ’; tissue culture; temperature; subculture cycle; light 0 引言 另一方面是研究培养