当归贝母苦参丸对良性前列腺增生模型小鼠PCNA-国际中医中药杂志.PDF

国际中医中药杂志2011年2月第33卷第2期 Int J Trad Chin Med, February 2011, Vol. 33, No.2 ·125 · ·实验研究 · 当归贝母苦参丸对良性前列腺增生模型 小鼠 PCNA 、bcl-2 和 bax 表达的影响 张奇峰 黄宗轩 高国政 贺铁豪 【摘要】 目的 探讨当归贝母苦参丸对良性前列腺增生（BPH ）模型小鼠的影响及作用机理。方 法 将 60 只雄性小鼠，按照随机数字表法随机分为 6 组：空白组，模型组，阳性对照组，高、中、低剂 量治疗组。空白组给予腹腔注射 12.5 mg/kg ·d－1橄榄油，其他各组采用腹腔注射丙酸睾酮原液制作小 鼠BPH模型，同时高、中、低剂量治疗组分别予高、中、低剂量当归贝母苦参丸水煎溶液灌胃，阳性对 照组灌胃前列康水混悬液 600 mg/kg 。给药第21 天处死，观察前列腺指数（PI ）的变化；HE染色光镜观 察前列腺病理改变；免疫组化方法检测PCNA 、bcl-2 和bax表达的变化。结果 当归贝母苦参丸治疗组PI 低于模型组；PCNA 、bcl-2 表达IOD值低于模型组；bax表达高于模型组。治疗组高、中、低剂量组比较 差异无统计学意义，三个治疗组与阳性对照组比较差异无统计学意义。结论 当归贝母苦参丸可抑制模型 小鼠BPH，其机理可能与下调细胞增殖蛋白PCNA和凋亡抑制蛋白bcl-2 、增加bax 的表达相关。 【关键词】 当归贝母苦参丸；良性前列腺增生 Effects of Danggui-beimu-kushen Pill on expression of PCNA, bcl-2 and bax in BPH mice model ZHANG Qi-feng, HUANG Zong-xuan, GAO Guo-zheng, HE Tie-hao. Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China Corresponding author：HE Tie-hao, Email ：hbb38@163.com 【Abstract 】 Objective To study the effect of Danggui-beimu-kushen Pill on benign prostatic hyperplasia (BPH) and its possible mechanism. Methods BPH model mouse was produced by intraperitoneal injection of testosterone propionate. The high, medium and low dose treatment group was fed high, middle and low dose of Danggui-beimu-kushen Pill respectively, while the positive control group was fed Qianliekang liquid, 600 mg/kg. All mice were executed on the 21 day. Such values were observed as the prostate index changes, pathological changes of prostate by HE staining light microscopy and the expression changes of PCNA, bcl-2 and bax by immunohistochemistry. Results The prostate index of the treatment group was lower than the model group; the PCNA and bcl