Notch4信号在小鼠胚胎早期造血发育的表达.doc

Notch4信号在小鼠胚胎早期造血发育的表达 张树君1 陈建斌1 杨春秀1 李芳菲2 姜蓉2 徐强3 （1 重庆医科大学附属第一医院血液内科 400016，2重庆医科大学干细胞与组织工程研究室 400016，3 重庆医科大学附属第一医院血管外科 400016） 目的：探讨小鼠胚胎发育过程早期造血组织中Notch4信号的基因与蛋白的表达情况。 方法：采用PT-PCR和 Western-blot 方法，对小鼠胚胎孕9.5天（embryonic day ,E9.5）、E10.5、E11.5、E12.5胚胎的主动脉-性腺-中肾（aorta-gonad-mesonephros , AGM）区和胎肝中Notch4信号基因和蛋白的表达进行检测。 结果：PT-PCR结果显示，Notch4 mRNA在AGM区表达较恒定，在胎肝中其表达量随孕龄增加而减少,E9.5表达最多。Notch4蛋白表达规律与Notch4 mRNA表达规律相同。 结论：Notch4信号在小鼠胚胎早期造血组织中有表达，但其功能有待进一步研究。 关键词：Notch4,主动脉-性腺-中肾区，胎肝，造血发育 基金项目：重庆市自然科学基金（2009BB5401） Expression of Notch4 in hematopoietic system during early mouse development Zhang shujun1 Chen jianbin1 Yang chunxiu1 Li fangfei2 Jiang rong2 Xu qiang3 （1The First Affiliated Hospital of Chongqing Medical University Department of Hematology 400016, 2 Chongqing Medical University ,Stem Cell and Tissue Engineering Research Laboratory 400016, 3 The First Affiliated Hospital of Chongqing Medical University Department of Vascular Surgery 400016） Objective: To investigate the expression of Notch4 gene and protein at the hematopoietic system in mouse early embryos. Methods: The expression of Notch4 mRNA and protein of the hematopoietic system from the mice embryonic aorta-gonad-mesonephros (AGM) region and fetal liver at embryonic day（E）9.5, E10.5,E11.5,E12.5 were detected by RT-PCR and Western-Blot techniques. Results: Notch4 mRNA showed a constant level of expression in AGM, and the expressions of Notch4 mRNA in Fetal liver were decreased with the pregnancy, with a peak at E9.5. The Notch4 protein expression showed the same laws as that of Notch4 mRNA. Conclusion: The Notch4 signals are expressed in early hematopotic tissue of mouse embryos, but its function is to be further researched. Keywords：Notch4，aorta-gonad-mesonephros (AGM), Fetal liver, Hematopoietic development Supported by the Natural Science Foundation of Chongqing (2009BB5401) （作者简介：张树君，女，1984年生，山东省临清市人，汉族，重庆医科大学附属第一医院血液科 硕士研究生。 HYPERLINK mailto:Zhangshujun2006@163.com Zhangshuju