剂量精确的大鼠无创气管滴注方法-中国试验动物学报.DOC

剂量精确的大鼠无创气管滴注方法 彭康琳1，周玉庆2，周正适3， （中南大学1.湘雅二医院心胸外科；2.湘雅三医院心内科；3.湘雅医学院实验动物学部，长沙410000) 【摘要】目的 建立一种滴注剂量精确的大鼠无创气管滴注方法。方法 选用一次性使用静脉输液针的胶管制作套管，透射光插管法行大鼠气管插管，微量进样器平头针经由套管插入气管进行滴注。滴注液体为生理盐水，滴注剂量5~200μL,精确度为1μL。结果 在体重200~350g的10只大鼠上共完成50次气管滴注操作，实验过程中和实验之后大鼠均无异常表现。结论 套管微量进样器法具有无创、精确、安全的特点，可广泛应用于大鼠经气管给药或染毒的各类研究。 【关键词】大鼠；气管滴注；方法 A high accuracy method for non-invasive intratracheal instillation in rats PENG Kang-lin1,ZHOU Yu-qing2, ZHOU Zheng-shi3 (1.Department of Cardiothoracic surgery,Second Xiangya Hospital;2.Department of Cardiology,Third Xiangya Hospital;3.Department of Laboratory Animal,Xiangya School of Medicine,Central South University,Changsha 410000,China) 【Abstract】Objective To develop a high accuracy method for non-invasive intratracheal instillation in rats. Methods The plastic catheter of disposable venous infusion needle was cut into a cannula,which was then intubated by transillumination.A microsyringe was introduced into the rat trachea through the cannula before the intratracheal instillation of saline solution was performed.The instilled volume ranged from 5μL to 200μL and the accuracy is 1μL. Results The intratracheal instillation operation was performed successfully for 50 times in 10 rats weighing 200~350g.The general health status was not affected during or after the operation in the rats.Conclusion The method developed in our study for intratracheal instillation is characterized by non-invasion, accuracy and safety,which could be widely used in HYPERLINK /dict/intratracheal intratracheal HYPERLINK /dict/drug drug administration or poisoning in rats. 【key words】 Rat;Intratracheal instillation;Method [作者简介]彭康琳（1985-），女，护师，本科，毕业于中南大学湘雅医学院护理系。 [通信作者]周正适。E-mail：zs.zhou@163.com 气管滴注是大鼠经气管给药或染毒的主要实验方法，其滴注剂量通常只有几十到几百微升，这种以微升计的精确性，普通注射器是无法达到的，只有微量进样器才符合要求。在有创的气管滴注[1]中，实验者可以切开大鼠颈部的皮肤和肌肉以暴露气管，再将尖头的微量进样器直接插入气管，从而做到精确滴注，但在无创的气管滴注中，实验者却不能使用微量进样器直接插入气管进行滴注，这是因为： = 1 \* GB3 ①无论平头或尖头的微量进样器相对于大鼠的口腔、咽喉和气管组织来说都过细过硬，容易损伤实验动物； = 2 \* GB3 ②微量进样器缺乏明确的气管插入标志； = 3 \* GB3 ③滴注完成之后