壳聚糖复合絮凝剂选择性絮凝双黄连水提取液的工艺研究.PDF

中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 13 期 2013 年 7 月 ·1761 · 壳聚糖复合絮凝剂选择性絮凝双黄连水提取液的工艺研究 Δ Δ Δ 白 研，周娉婷 ，潘杰谦 ，陈亮红 广东药学院公共卫生学院，广东 广州 510310 摘 要：目的 优化壳聚糖复合絮凝剂用于双黄连水提取液选择性絮凝的最佳工艺条件。方法 以绿原酸和黄芩苷保留率、 鞣质脱除率为考察指标，分别考察复合絮凝剂制备工艺、絮凝剂用量、絮凝时间以及壳聚糖性能指标对絮凝效果的影响，优 化选择性絮凝工艺条件。结果 双黄连水提取液絮凝的优化工艺条件：膨润土经 450 ℃灼烧4 h 改性后负载壳聚糖制备复合 絮凝剂，絮凝剂用量 60 g/L，絮凝时间24 h ，所用壳聚糖最佳性能指标：脱乙酰度 95%、黏度 60 cps 。结论 经高温改性的 膨润土负载壳聚糖所制备的复合絮凝剂具有良好的选择性絮凝性能，絮凝效果显著优于《中国药典》2010 年版方法。 关键词：壳聚糖；膨润土；复合絮凝剂；双黄连口服液；绿原酸；黄芩苷；鞣质 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2013)13 - 1761 - 07 DOI: 10.7501/j.issn.0253-2670.2013.13.012 Selective flocculation processing technology of chitosan complex flocculant on Shuanghuanglian aqueous extract BAI Yan, ZHOU Ping-ting, PAN Jie-qian, CHEN Liang-hong School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China Abstract: Objective To optimize the selective flocculation processing technology of chitosan complex flocculant on Shuanghuanglian aqueous extract. Methods The impacts of preparation technology, the dosage of complex flocculant, the time of flocculation, and the performance index of chitosan on the flocculating effect of Shuanghuanglian aqueous extract were examined by using the retention rates of chlorogenetic acid and baicalin, as well as the elimination rate of tannin as observation indicators. Results The selective flocculation processing technology on Shuanghuanglian aqueous extract was optimized as follows: the bentonite was loaded by chitosan after it was burned for 4 h at 450 ℃, the dosage of complex flocculant was 60 g/L, the flocculating time was 24 h, and the best performance index of chitosan is 95% in deacetylation, and 60 cps in viscosity. Conclusion The complex flocculant made of high temperature modified bentonite loaded by chi