双歧杆菌脂磷壁酸对B16荷瘤小鼠FasFasL表达的影响.docVIP

双歧杆菌脂磷壁酸对B16荷瘤小鼠Fas/FasL表达的影响 李芙蓉1，作者简介：李芙蓉，女，讲师，主要从事肿瘤免疫方面的研究。现在贵州省遵义医学院免疫教研室工作。电话：（0852）8609530， 作者简介：李芙蓉，女，讲师，主要从事肿瘤免疫方面的研究。现在贵州省遵义医学院免疫教研室工作。电话：（0852）8609530， Email:lfr-136@163.com 通信作者：王跃， Tel: （028Email: wangyue_26@ 收稿日期：2009-11-26；修回日期：2010-02-28 （1遵义医学院免疫教研室，2成都医学院检验系，成都 610031 ） 摘要: 目的 探讨双歧杆菌脂磷壁酸（BLTA）对黑色素瘤B16小鼠Fas/FasL表达的影响。 方法 将黑色素瘤B16细胞接种于C57BL/6小鼠皮下，待触及肿块后于荷瘤小鼠瘤周注射不同剂量的BLTA。 采用MTT法检测荷瘤小鼠CTL杀伤活性；免疫组织化学染色检测肿瘤组织浸润的CD4+ 、CD8+ 淋巴细胞的表达情况；用RT-PCR检测肿瘤组织和脾脏组织Fas、FasL mRNA 的表达变化；免疫组织化学染色和western blot检测肿瘤组织和脾脏淋巴细胞Fas、FasL 蛋白的表达变化。结果 与对照组相比，BLTA处理组荷瘤小鼠的CTL杀伤率明显增加（P0.05），肿瘤组织内Fas、FasL mRNA和蛋白表达明显增加（P0.05），而荷瘤小鼠脾脏组织的Fas 、FasL mRNA和蛋白表达明显下调（P0.05），肿瘤组织内CD8+淋巴细胞的浸润增多（P0.05），并具有剂量依赖性。 CD4+淋巴细胞的浸润无明显增加（P0.05）。 结论 BLTA能够通过调节Fas/FasL系统，增强CTL细胞的杀伤活性，促进肿瘤细胞的凋亡，逆转荷瘤小鼠的肿瘤免疫逃逸现象，进而提高机体的抗肿瘤作用。 关键词：双歧杆菌；脂磷壁酸；黑色素瘤；Fas；FasL 中图分类号：R730.51 文献标识码：A Effects of lipoteichoic acid of bifidobacterium on expression of Fas/FasL in B16 tumor-bearing mice Abstract：Objective To investigate the effect of lipoteichoic acid of Bifidobacterium(BLTA) on the expressions of Fas/FasL in bearing melanoma B16 cancer mice. Methods Melanoma B16 cells were subcutaneously injected into C57BL/6 mice, BLTA was injected subcutaneously into the mice when the tumor was palpable. The changes of the killing activity of CTL was examined by MTT assay; the expression of the tumor-infiltrating CD4+ and CD8+ lymphocytes in tumor tissue were detected by immunohistochemistry; RT-PCR was used to detect the mRNA expression variance of the Fas and FasL in tumor and spleen tissue; and the protein expression of Fas, FasL in tumor tissue and lymphocytes of spleen were detected by immunohistochemistry and western blot. Results Compared with the control group, BLTA can significantly promote the CTL killing activity (P0.05); the mRNA and protein expression of Fas, FasL were significantly up-regulated in the tumor tissue (P0.05) whereas it were down regulated in spleen tissue (P0.05), CD8+ lymphocy