双氢青蒿素对前列腺癌裸鼠种植瘤生长及VEGF表达的影响.docVIP

双氢青蒿素对前列腺癌裸鼠种植瘤生长及VEGF表达的影响 李庆春 高小玲 石之虎 邹 聪 罗子国（重庆医科大学电镜室，重庆 400016） 摘要 目的 研究双氢青蒿素（DHA）对前列腺癌PC-3细胞裸鼠种植瘤的抑制作用及对VEGF表达的影响，并探讨其作用机制。方法 采用人前列腺癌PC-3细胞建立裸鼠种植瘤模型，20只模型鼠随机分为对照组、溶剂组、DHA大剂量组及DHA小剂量组，经13d干预后，计算抑瘤率；光镜及电镜观察肿瘤组织形态学表现，免疫组织化学法检测VEGF的表达水平，体视学分析肿瘤微血管密度（MVD）。结果 DHA大小剂量组抑瘤率分别为69.221%和63.186%，肿瘤组织内凋亡细胞明显增多，可见典型的凋亡小体；免疫组化结果显示，DHA大小剂量组VEGF表达及MVD均低于对照组（P＜0.05）。结论 DHA具有较强的抗肿瘤作用，其机制与下调凋亡相关基因VEGF有关。 关键词: 双氢青蒿素；前列腺癌；凋亡；VEGF；微血管密度 中图法分类号：R284.1;R979.1 文献标识码：A The effects of dihydroartemisinin on transplanted prostate cancer in nude mice and expression of VEGF LI Qing-chun, GAO Xiao-lin, SHI Zhi-hu，ZOU Cong ,LUO Zi-guo* (Laboratory of Electron Microscope, Chongqing Medical University, Chongqing 400016, China) [Abstract] Objective:To study the inhibitory effects of dihydroartemisinin(DHA) on the growth of transplantation human prostate tumor PC-3 cells in nude mice and the expression level of VEGF and explore the underlying action mechanism of DHA. Methods: Prostate cancer PC-3 cells were transplanted into 20 nude mice to establish the solid tumor mode. These nude mice were randomly divided into 4 groups with 5 mice in each group: control group , solvent group, large dose DHA group and low dose DHA group. The tumor growth inhibition rate was calculated on day 13 after drug administration;Pathomorphism changes of PC-3 cells were observed by light microscope(LM) and transmission electron microscope(TEM) after administration of DHA. The protein expression of VEGF was tested by immunohistochemical method; 作者简介：李庆春，河北省张家口市人，男，医师，主要从事前列腺癌方面的研究。电话E-mail :695786349@ 通信作者：罗子国，电话：（023E-mail : luoziguo@ 收稿日期：08-07-11 修回日期：08-08-31 Micro-vasvulor density(MVD) was analysed by stereological method. Results: The tumor growth inhibition rates of large dose DHA group and low dose DHA group were 69.221% and 63.186%; Apoptotic cells were significantly increased and TEM examination revealed that the scattered apoptotic bodies were observed in tumor tissues of DHA g