口腔鳞癌组织中OmiHtrA2的表达及临床意义.docVIP

PAGE 4 口腔鳞癌组织中Omi/HtrA2的表达及临床意义 陈彦平1 黑乃恒1 马景枝2 梁玉林3 杨会钗 4王小玲4 （050011 石家庄， 河北医科大学第四医院：口腔科1，检验科2 病理科4;051430 石家庄，河北省栾城县医院口腔科3） [提要] 目的: 检测Omi/HtrA2 在口腔鳞癌组织中的表达, 初步探讨其在口腔鳞癌发生、发展中的作用及意义。方法: 采用RT-PCR与免疫组织化学方法, 检测20 例口腔低分化鳞状细胞癌、20 例口腔高分化鳞状细胞癌、20例口腔鳞状上皮异型增生组织、20例癌旁组织切除标本中Omi/HtrA2 mRNA 与蛋白的表达情况，并与正常组织标本中Omi/HtrA2 mRNA 与蛋白的表达情况作比较。结果: 正常组织中未检测到Omi/HtrA2 蛋白表达, 而在口腔鳞癌组织与口腔鳞状上皮异型增生组织中均有表达(P0.05)。Omi/HtrA2 基因在高分化口腔鳞癌组织中的表达水平高于低分化型口腔鳞癌组织、异型增生组织以及癌旁和正常组织（P0.05）。结论: Omi/HtrA2 在口腔鳞状细胞癌组织中有表达, 表明Omi/HtrA2 的促凋亡作用参与了口腔鳞癌的发生发展过程, 为口腔鳞癌治疗提供了新的理论基础。 [关键词] Omi/HtrA2； 口腔鳞状细胞癌 ； 逆转录聚合酶链式反应 ； 免疫组织化学方法 [中图法分类号] R：739.85 [文献标识码] A The Expression of Omi/HtrA2 in oral squamons cell carcinoma Chen Yanping1 Hei Naiheng1 Liang Yulin3 Yang Huichai4 Wang Xiaoling4(050011 Shi Jiazhuang,The Fourth Hospital of Hebei Medical University:Department of Stomatology1,Blood screening test department2，Department of Pathology4;051430 Shi jiazhuang ,Department of Stomatology,The LuanCheng Hospital of the He Bei Province3) [Abstract] Objective: To investigate the expression of the Omi/HtrA2 and its significance in carcinogenesis and development of the 0ral squamons cell carcinoma. Methods: The expressions of Omi/HtrA2 mRNA and protein were determined using immunohistochemistry and semi- quantitative reverse transcription polymerase chain reaction in 40 cases with Oral squamons cell carcinoma(well and poor differentiation of each half)、20 with Epithelial dysplasia、20 with paracancerous tissues and 20 nomal tissues. Results: No immunoreactivity of Omi/HtrA2 was observed in the nomal tissues, but there was an evident Omi/HtrA2 immunoreactivity in Oral squamons cell carcinoma and Epithelial dysplasia, which showed no significant difference between them(P0.05) . The expression of Omi/HtrA2 mRNA in well differentiated Oral squamons cell carcinoma ca