柠檬酸杆菌对长期铅暴露小鼠致死率及外周血炎症因子的影响.doc

PAGE PAGE 6 HYPERLINK mailto:androtin@ androtin@ 金项目：新乡医学院重点研究领域招标课题（ZD2011-15 基金项目：新乡医学院重点研究领域招标课题（ZD2011-15）；新乡医学院大学生创新课题资助计划 作者简介：黄青松（1978-），女，讲师，从事免疫毒理学研究，xxmuhqs@163.com。 通讯作者：牛志国，androtin@ 柠檬酸杆菌对长期铅暴露小鼠致死率及外周血炎症因子的影响 黄青松1，牛志国2*，孙书明2，孙启蒙3，赵晓楠3，牛艳华3，聂慧岩3，魏小辉3 新乡医学院1.微生物学教研室；2.免疫学教研室；3.临床医学系；河南 新乡 453003；*通讯作者 摘要：目的 探讨柠檬酸杆菌对长期铅暴露小鼠致死率及外周血炎症因子的影响。方法 将40只健康初断乳21日龄清洁级雄性KM小鼠随机分为4组：对照（蒸馏水）组和低（200 mg/L）、中（400 mg/L）、高（800 mg/L）剂量乙酸铅染毒组，每组10只。采用自由饮水方式进行染毒，连续染毒12周。染毒结束后，1×109 CFU的柠檬酸杆菌灌胃，计算7d死亡率；ELISA检测外周血IL-6、TNF-α、IFN-γ及IL-22。结果 与对照组相比，各剂量染铅组小鼠腹腔注射柠檬酸杆菌后，死亡率明显升高，差异具有统计学意义(P＜0.05)；与对照组相比，各剂量染铅组小鼠腹腔注射柠檬酸杆菌后，外周血IL-6、TNF-α、IFN-γ、IL-22的水平明显降低，差异均具有统计学意义(P＜0.05)。随着染铅剂量升高，小鼠注射柠檬酸杆菌后，外周血外周血IL-6、TNF-α、IFN-γ、IL-22的水平呈现下降趋势，而死亡率呈现上升趋势。结论：长期铅暴露可导致小鼠的黏膜抗感染能力下降。 关键词：金属，铅；弗氏柠檬酸杆菌；炎症因子；致死率 中图分类号：R994.6 文献标志码：A The influence of LPS on the mortality and peripheral inflammatory factors in mice with Long-term Exposure to Lead QingSong Huang1, ZhiGuo Niu2*, Shuming Sun2,Qimeng Sun3, ZhaoXiao Nan3, YanHua Niu3, HuiYan Nie3, XiaoHui Wei 1 The Department of Microbiology; 2 The Department of Immunology; 3 Undergraduate of College of Preclinical Medicine; Xinxiang medical university, Xinxiang, Henan, 453003, China；* Corresponding author Abstract：Objective To study the median lethal dose of Citrobacter freundii to mice mortality rate and peripheral blood inflammatory factors with long-term lead exposure.. Methods A total of 40healthy and cleanly male KM mice weaned at 21 days were randomly and evenly divided into four groups，named control group (distilled water) and low (200 mg/L)，medium (400 mg/L)，high (800 mg/L) dose of lead acetate exposure group. Free access to water were continuously exposed to lead acetate for 12 weeks. When exposed to the end, the other group of mice by gavaged injection of 1×109CFU Citrobacter freundii, Peripheral blood IL-6, TNF-alpha and IFN-γ and of IL-22 levels were determined by ELISA. Results Compared with control grou